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Author Notes:

Correspondence: Nicholas M. Boulis, M.D., 101 Woodruff Circle, Room 6339, Atlanta, Georgia 30322, USA. Telephone: 404‐727‐2247; e‐mail: nboulis@emory.edu; or Jason J. Lamanna, Ph.D., 101 Woodruff Circle, Room 6339, Atlanta, Georgia 30322, USA. Telephone: 404‐727‐2247; e‐mail: jlamann@emory.edu

J.J.L.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript; J.G. and L.N.U.: collection and/or assembly of data, data analysis and interpretation; C.V.H., E.A., and N.G.: collection and/or assembly of data; C.N.S.: financial support, provision of study material or patients; T.F.: conception and design, financial support, administrative support, collection and/or assembly of data, data analysis and interpretation, final approval of manuscript; J.N.O.: conception and design, administrative support, collection and/or assembly of data, data analysis and interpretation, final approval of manuscript; N.M.B.: conception and design, financial support, administrative support, provision of study material or patients, data analysis and interpretation, final approval of manuscript.

J.J.L.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript; J.G. and L.N.U.: collection and/or assembly of data, data analysis and interpretation; C.V.H., E.A., and N.G.: collection and/or assembly of data; C.N.S.: financial support, provision of study material or patients; T.F.: conception and design, financial support, administrative support, collection and/or assembly of data, data analysis and interpretation, final approval of manuscript; J.N.O.: conception and design, administrative support, collection and/or assembly of data, data analysis and interpretation, final approval of manuscript; N.M.B.: conception and design, financial support, administrative support, provision of study material or patients, data analysis and interpretation, final approval of manuscript.

The other authors indicated no potential conflicts of interest.

Subjects:

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Cell & Tissue Engineering
  • Cell Biology
  • Cell transplantation
  • Clinical translation
  • In vivo tracking
  • Neural stem cell
  • Pig model
  • Stem cell transplantation
  • Magnetic resonance imaging
  • Spinal cord
  • AMYOTROPHIC-LATERAL-SCLEROSIS
  • MESENCHYMAL STEM-CELLS
  • REGENERATIVE MEDICINE
  • STROMAL CELLS
  • CLINICAL-USE
  • RAT-BRAIN
  • TRANSPLANTATION
  • MODEL
  • IRON
  • MRI

Ferumoxytol Labeling of Human Neural Progenitor Cells for Diagnostic Cellular Tracking in the Porcine Spinal Cord With Magnetic Resonance Imaging

Tools:

Journal Title:

Stem Cells Translational Medicine

Volume:

Volume 6, Number 1

Publisher:

, Pages 139-150

Type of Work:

Article | Final Publisher PDF

Abstract:

We report on the diagnostic capability of magnetic resonance imaging (MRI)-based tracking of ferumoxytol-labeled human neural progenitor cells (hNPCs) transplanted into the porcine spinal cord. hNPCs prelabeled with two doses of ferumoxytol nanoparticles (hNPC-F Low and hNPC-F High ) were injected into the ventral horn of the spinal cord in healthy minipigs. Ferumoxytol-labeled grafts were tracked in vivo up to 105 days after transplantation with MRI. Injection accuracy was assessed in vivo at day 14 and was predictive of “on” or “off” target cell graft location assessed by histology. No difference in long-term cell survival, assessed by quantitative stereology, was observed among hNPCF Low , hNPC-F High , or control grafts. Histological iron colocalized with MRI signal and engrafted human nuclei. Furthermore, the ferumoxytol-labeled cells retained nanoparticles and function in vivo. This approach represents an important leap forward toward facilitating translation of cell-tracking technologies to clinical trials by providing a method of assessing transplantation accuracy, delivered dose, and potentially cell survival.

Copyright information:

© 2016 The Authors.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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