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Author Notes:

Address correspondence to: Jihui Ren (jihuiren2012@gmail.com), Vytas A. Bankaitis (vytas@tamhsc.edu).

We thank Chao-wen Wang (Institute of Plant and Microbial Biology, Taiwan) for Pet10 antibody and Henrik Dohlman (University of North Carolina at Chapel Hill) for kindly facilitating the pulse-labeling experiments.

We are also grateful to Michael Kay (University of Utah, Salt Lake City, UT) and Joel Goodman (UT–Southwestern Medical Center, Dallas, TX) for helpful discussions and comments and Lora Yanagisawa (Texas A&M Health Science Center, College Station, TX) for critical comments on the manuscript.

We acknowledge the University of North Carolina Lineberger Comprehensive Cancer Center Genome Analysis and Nucleic Acids Core facilities.

The authors declare no financial conflicts.

Subjects:

Research Funding:

This work was supported by Grant GM44530 from the National Institutes of Health (V.A.B.) and the Robert A. Welch Foundation (V.A.B.). J.R. was supported by a postdoctoral fellowship from the American Heart Association (11POST6880007).

A.M.N. was supported by National Institutes of Health Grant GM72540, D.M.E. and the University of Utah Protein Interactions Core Facility were supported by National Institutes of Health Grant GM82545.

T.J.L. and R.C.M. were supported by National Institutes of Health Grant GM069338, and P.T.I., D.S.M., and H.A.B. were supported by National Institutes of Health U54 GM069338 and PO1 ES013125.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Cell Biology
  • CELL BIOLOGY
  • PHOSPHOLIPASE-D ACTIVITY
  • HEPATITIS-C VIRUS
  • IONIZATION MASS-SPECTROMETRY
  • HORMONE-SENSITIVE LIPASE
  • SACCHAROMYCES-CEREVISIAE
  • ENDOPLASMIC-RETICULUM
  • MEDIATED LIPOLYSIS
  • CELL-GROWTH
  • PERILIPIN-A
  • YEAST SAC1

A phosphatidylinositol transfer protein integrates phosphoinositide signaling with lipid droplet metabolism to regulate a developmental program of nutrient stress-induced membrane biogenesis

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Journal Title:

Molecular Biology of the Cell

Volume:

Volume 25, Number 5

Publisher:

, Pages 712-727

Type of Work:

Article | Final Publisher PDF

Abstract:

Lipid droplet (LD) utilization is an important cellular activity that regulates energy balance and release of lipid second messengers. Because fatty acids exhibit both beneficial and toxic properties, their release from LDs must be controlled. Here we demonstrate that yeast Sfh3, an unusual Sec14-like phosphatidylinositol transfer protein, is an LD-associated protein that inhibits lipid mobilization from these particles. We further document a complex biochemical diversification of LDs during sporulation in which Sfh3 and select other LD proteins redistribute into discrete LD subpopulations. The data show that Sfh3 modulates the efficiency with which a neutral lipid hydrolase-rich LD subclass is consumed during biogenesis of specialized membrane envelopes that package replicated haploid meiotic genomes. These results present novel insights into the interface between phosphoinositide signaling and developmental regulation of LD metabolism and unveil meiosis-specific aspects of Sfh3 (and phosphoinositide) biology that are invisible to contemporary haploid-centric cell biological, proteomic, and functional genomics approaches.

Copyright information:

© 2014 Ren et al.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License (http://creativecommons.org/licenses/by-nc-sa/3.0/).

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