About this item:

609 Views | 195 Downloads

Author Notes:

Corresponding author. Mailing address: Department of Chemistry, 1515 Dickey Drive, Emory University, Atlanta, GA 30322. Phone: (404) 712-2171. Fax: (404) 727-6586. E-mail: justin.gallivan@emory.edu

S.T., C.M.K.R., J.C.S., I.S.G., S.K.D., D.M., A.S., and A.W.P. designed and performed experiments.

J.P.G., J.R.S., C.R.B., and A.K. provided oversight.

S.T. and J.P.G. conceived the experiments and wrote the paper.

We are grateful to Anton Bryksin and Ichiro Matsumura for plasmids, strains, and helpful discussions.

The technical advice of Barbara Froehlich and Julia Bugrysheva for work with S. pyogenes is appreciated.

S.T. and S.K.D. were G.W. Woodruff Fellows.

S.T. is an Irvington Institute Fellow of the Cancer Research Institute.

A.S. is an NIH K99 Postdoctoral Fellow (K99GM092934).

A.W.P. is an NSF Predoctoral Fellow.

A.K. is a David and Lucille Packard Foundation Fellow.

J.P.G. is an Alfred P. Sloan Fellow and a Camille Dreyfus Teacher-Scholar.

Subjects:

Research Funding:

This work was supported in part by NIH grants (GM074070 to J.P.G., AI20723 to J.R.S., GM084122 to A.K., and AI51622 to C.R.B.).

Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species▿ †

Show all authors Show less authors

Tools:

Journal Title:

Applied and Environmental Microbiology

Volume:

Volume 76, Number 23

Publisher:

, Pages 7881-7884

Type of Work:

Article | Final Publisher PDF

Abstract:

We developed a series of ligand-inducible riboswitches that control gene expression in diverse species of Gram-negative and Gram-positive bacteria, including human pathogens that have few or no previously reported inducible expression systems. We anticipate that these riboswitches will be useful tools for genetic studies in a wide range of bacteria.

Copyright information:

© 2010, American Society for Microbiology

Export to EndNote