About this item:
579 Views | 694 Downloads
Author Notes:
Correspondence: E-mail: SSable@cdc.gov
Subhadra Nandakumar, Sunil Kannanganat, and Suraj B. Sable contributed equally to this work.
Conceived and designed the experiments: SBS SN SK KMD RRA.
Performed the experiments: SN SBS SK KB MVR JEP JSS KMD.
Analyzed the data: SK SN SBS KMD VC.
Contributed reagents/materials/analysis tools: KMD ML JSS SF MAM JP BBP RRA.
Wrote the paper: SBS KMD JP JEP.
The authors thank M. Iademarco and T.M. Shinnick for helpful discussions, S. Malik, M. Willby, and A. Zaunbrecher (Division of Tuberculosis Elimination, CDC) for assistance with CFU enumeration experiments, Mary Leonhardt and Harrison Huntoon (Colorado State University, Fort Collins, CO) for IL-2 assays using 4C3 clone and nApa trypsin digestion, and Amy Yang, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, for providing preclinical BCG vaccine and Mtb Erdman challenge standards.
The findings and conclusions in this publication are those of the authors and do not necessarily represent the official position of CDC, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.
The authors have declared that no competing interests exist.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Subjects:
Research Funding:
This work was supported by Georgia Research Alliance (to SBS and RRA as principle investigators and JEP and TM Shinnick as co-investigators), National Institute of Health/National Institute of Allergy and Infectious Diseases contract HHSN266200400091c (to KMD), and Centers for Disease Control and Prevention intramural grants.
SN and MVR acknowledge the postdoctoral financial assistance from ORISE and Emerging Infectious Diseases fellowship, respectively.
Keywords:
- Science & Technology
- Life Sciences & Biomedicine
- Microbiology
- Parasitology
- Virology
- MICROBIOLOGY
- PARASITOLOGY
- VIROLOGY
- FIBRONECTIN ATTACHMENT PROTEIN
- DENDRITIC CELLS
- MANNOSE RECEPTOR
- 45-KILODALTON GLYCOPROTEIN
- IMMUNE-RESPONSES
- PRESENTING CELLS
- TRANSGENIC MICE
- VACCINE DESIGN
- B-CELL
- GLYCOSYLATION
- Cytokines
- T cells
- Mycobacterium tuberculosis
- Vaccination and immunization
- Antigens
- Spleen
- Cytotoxic T cells
- Enzyme-linked immunoassays
O-mannosylation of the Mycobacterium tuberculosis Adhesin Apa Is Crucial for T Cell Antigenicity during Infection but Is Expendable for Protection
Abstract:
Copyright information:
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
