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Author Notes:

E-mail: lamcgraw@ncsu.edu

Conceived and designed the experiments: LAM LJY JWT.

Performed the experiments: JKD NISC.

Analyzed the data: LAM PJT JWT.

Wrote the paper: LAM JWT.

The authors wish to acknowledge Greg K. Tharp for computational support, the Georgia Research Alliance Genomics Core for the 454 sequencing, and members of the NIH Intramural Sequencing Center, including E. D. Green, R. Blakesley, G. Bouffard, J. Mullikin, and J. McDowell.

The authors have declared that no competing interests exist.


Research Funding:

The NIH Intramural Sequencing Center was supported by the Intramural Research Program of the National Human Genome Research Institute (www.genome.gov/) of the National Institutes of Health (www.nih.gov).

JWT, JKD, and LJY were supported by National Institutes of Health grant 1R21MH082225 and LAM by grant 1F32MH079661.

LJY was further supported by NIH MH064692, Autism Speaks (www.autismspeaks.org) and RR00165 to YNPRC.


  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics

BAC-Based Sequencing of Behaviorally-Relevant Genes in the Prairie Vole

Journal Title:



Volume 7, Number 1


, Pages e29345-e29345

Type of Work:

Article | Final Publisher PDF


The prairie vole (Microtus ochrogaster) is an important model organism for the study of social behavior, yet our ability to correlate genes and behavior in this species has been limited due to a lack of genetic and genomic resources. Here we report the BAC-based targeted sequencing of behaviorally-relevant genes and flanking regions in the prairie vole. A total of 6.4 Mb of non-redundant or haplotype-specific sequence assemblies were generated that span the partial or complete sequence of 21 behaviorally-relevant genes as well as an additional 55 flanking genes. Estimates of nucleotide diversity from 13 loci based on alignments of 1.7 Mb of haplotype-specific assemblies revealed an average pair-wise heterozygosity (8.4×10 -3 ). Comparative analyses of the prairie vole proteins encoded by the behaviorally-relevant genes identified > 100 substitutions specific to the prairie vole lineage. Finally, our sequencing data indicate that a duplication of the prairie vole AVPR1A locus likely originated from a recent segmental duplication spanning a minimum of 105 kb. In summary, the results of our study provide the genomic resources necessary for the molecular and genetic characterization of a high-priority set of candidate genes for regulating social behavior in the prairie vole.

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an Open Access work distributed under the terms of the Creative Commons Universal : Public Domain Dedication License (http://creativecommons.org/publicdomain/zero/1.0/).

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