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Author Notes:

Corresponding Author: Cherry Wongtrakool, MD, Division of Pulmonary, Allergy, and Critical Care Medicine, 615 Michael Street, Suite 205, Atlanta, GA 30322, cwongtr@emory.edu, Phone: (404)712-2970, FAX: (404)712-2974

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Research Funding:

Funded by National Institutes of Health Grant K08 HL080293 (to C.W.) and Children’s Center for Developmental Lung Biology (to T.W.G., L.A.B.).

In Utero Nicotine Exposure Promotes M2 Activation in Neonatal Mice Alveolar Macrophages

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Journal Title:

Pediatric Research

Volume:

Volume 72, Number 2

Publisher:

, Pages 147-153

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Background Maternal smoking in utero has been associated with adverse health outcomes including lower respiratory tract infections in infants and children, but the mechanisms underlying these associations continue to be investigated. We hypothesized that nicotine plays a significant role in mediating the effects of maternal tobacco smoke on neonatal alveolar macrophage (AM) function, the resident immune cell in the neonatal lung. Methods Primary AMs were isolated at postnatal day 7 from a murine model of in utero nicotine exposure. The murine AM cell line MH-S was used for additional in vitro studies. Results In utero nicotine increased IL-13 and transforming growth factor beta one (TGFβ1) in the neonatal lung. Nicotine-exposed AMs demonstrated increased TGFβ1 and increased markers of alternative activation with diminished phagocytic function. However, AMs from mice deficient in the α7 nicotinic acetylcholine receptor (α7 nAChR) had less TGFβ1, reduced alternative activation and improved phagocytic functioning despite similar in utero nicotine exposure. Conclusion In utero nicotine exposure, mediated in part via the α7 nAChR, may increase the risk of lower respiratory tract infections in neonates by changing the resting state of AM towards alternative activation. These findings have important implications for immune responses in the nicotine-exposed neonatal lung.

Copyright information:

© 2012 International Pediatric Research Foundation, Inc.

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