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Author Notes:

Correspondence: E-mail: skumar@knights.ucf.edu

Conceived and designed the experiments: SK GPN.

Performed the experiments: SK GPN.

Analyzed the data: SK GPN.

Contributed reagents/materials/analysis tools: SK DWB HS LV.

Wrote the paper: SK.

Provided guidance for the research, worked further on data analysis, and critically reviewed the manuscript: HS LV.

Principal investigator of the project under NSF DBI-0442412: DWB.

This study is dedicated to the memory of Dr. David Borst who passed away on September 27, 2010.

Sandeep Kumar is grateful for his wonderful support and guidance.

He was a brilliant teacher and researcher and will always be remembered.

The contents are solely the responsibility of the authors and do not necessarily represent the official views of NSF.

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests: The authors have declared that no competing interests exist.

Subjects:

Research Funding:

This research was supported by a grant from National Science Foundation, NSF-0611447 (DW Borst).

Keywords:

  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics
  • MULTIDISCIPLINARY SCIENCES
  • BLOWFLY CALLIPHORA-VOMITORIA
  • RED SWAMP CRAYFISH
  • METHIONINE-ENKEPHALIN
  • OVARIAN MATURATION
  • IN-VIVO
  • MET-ENKEPHALIN-ARG6-GLY7-LEU8-LIKE PEPTIDES
  • FUNCTIONAL-SIGNIFICANCE
  • PROCAMBARUS-CLARKII
  • NEURONAL PATHWAYS
  • JUVENILE-HORMONE
  • Crustaceans
  • Grasshoppers
  • Insect physiology
  • Insects
  • Oocytes
  • Reproductive physiology
  • Gonads
  • Opioids

Exposure to Exogenous Enkephalins Disrupts Reproductive Development in the Eastern Lubber Grasshopper, Romalea microptera (Insecta: Orthoptera)

Tools:

Journal Title:

PLoS ONE

Volume:

Volume 7, Number 11

Publisher:

, Pages e51126-e51126

Type of Work:

Article | Final Publisher PDF

Abstract:

Enkephalins play a major role in reproductive physiology in crustaceans; however their role in reproductive development in insects is largely unknown. We investigated the effect of exposure to exogenous leucine-enkephalin (Leu-Enk), methionine-enkephalin (Met-Enk), and the opioid antagonist naloxone on gonad development in the Eastern lubber grasshopper, Romalea microptera. Injection of either Leu-Enk or naloxone alone significantly increased the testicular index and testicular follicular diameter in males, and the ovarian index, oocyte length, and oocyte diameter in females. In contrast, injection of Met-Enk inhibited all measures of reproductive development in both sexes. Surprisingly, co-injection of naloxone with either enkephalin enhanced the effect associated with administration of the enkephalin alone. This study clearly demonstrates the ability of enkephalins to disrupt insect sexual development and also suggests the existence of conserved enkephaline-dependent regulatory mechanisms in insects and crustaceans.

Copyright information:

© 2012 Kumar et al.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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