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Author Notes:

Yoshinori Marunaka, Email: marunaka@koto.kpu-m.ac.jp

T.No., A.T. and M.Sh. performed the experiments and the analytical part of the study.

Y.M., T.No. and A.T. designed the experiments. T.No. and Y.M. wrote the manuscript.

T.No., A.T., T.Na., T.I., M.So., D.C.E., and Y.M. discussed the observations and provided explanation on the observations.

Y.M. supervised the project.

All authors reviewed the manuscript.

T.No. and A.T. are co-first authors.

The authors declare that they have no competing interests.

Subjects:

Research Funding:

This work was supported by Grants-in-Aid from Japan Society of the Promotion of Science (JSPS KAKENHI Grant Number JP26460300 to TN, JP26713008 and JP16K15181 to AT, JP25670111 and JP15K15034 to YM), US NIH NIDDK R37 DK037963 to DCE, Salt Science (1235 to YM, 1429 and 1542 to AT), KIT-KPUM-KPU-KPhU Collaborative Research Grant (2013 and 2015) to YM, Society for Research on Umami Taste, Nestlé Nutrition Council, Japan to AT, Kyoto Prefectural Public University Corporation to AT, Kyoto-Funding for Innovation in Health-related R&D Fields to YM, Fuji Foundation for Protein Research to YM, and Cell Research Conference to YM.

Keywords:

  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics
  • GAP-JUNCTION PROTEINS
  • PERMEATION PORE INHIBITION
  • INDUCED NEURITE OUTGROWTH
  • FIND-ME SIGNAL
  • CELL-PROLIFERATION
  • POTASSIUM CHANNELS
  • RECEPTOR-CHANNEL
  • CONNEXIN FAMILY
  • CANCER CELL
  • ATP RELEASE

Current-direction/amplitude-dependent single channel gating kinetics of mouse pannexin 1 channel: a new concept for gating kinetics

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Journal Title:

Scientific Reports

Volume:

Volume 7, Number 1

Publisher:

, Pages 10512-10512

Type of Work:

Article | Final Publisher PDF

Abstract:

The detailed single-channel gating kinetics of mouse pannexin 1 (mPanx1) remains unknown, although mPanx1 is reported to be a voltage-activated anion-selective channel. We investigated characteristics of single-channel conductances and opening and closing rates of mPanx1 using patch-clamp techniques. The unitary current of mPanx1 shows outward rectification with single-channel conductances of ~20 pS for inward currents and ~80 pS for outward currents. The channel open time for outward currents (Cl(-) influx) increases linearly as the amplitude of single channel currents increases, while the open time for inward currents (Cl(-) efflux) is constant irrespective of changes in the current amplitude, as if the direction and amplitude of the unitary current regulates the open time. This is supported by further observations that replacement of extracellular Cl(-) with gluconate(-) diminishes the inward tail current (Cl(-) efflux) at a membrane potential of -100 mV due to the lowered outward current (gluconate(-) influx) at membrane potential of 100 mV. These results suggest that the direction and rate of charge-carrier movement regulate the open time of mPanx1, and that the previously reported voltage-dependence of Panx1 channel gating is not directly mediated by the membrane potential but rather by the direction and amplitude of currents through the channel.

Copyright information:

© The Author(s) 2017

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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