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Author Notes:

Corresponding authors: Haian Fu hfu@emory.edu and Xu Zhang

We thank members of the Fu lab, Emory University and Jiangsu key laboratory for TCM formulae research, Nanjing University of Chinese Medicine for assistance and enlightening discussions.

Competing Interests: The authors have declared that no competing interest exists.

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Research Funding:

This work was supported in part by National Science & Technology Pillar Program in the 11th Five-year Plan of China 2006BAI11B08-01(to H.F., and X.Z), the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions (to X.Z), the Research and Innovation Program of Postgraduates in Jiangsu Province (to M.C.).

Technology Pillar Program in the 11th Five-year Plan of China 2006BAI 11B08-01 (to H.F. and X.Z.), the U.S. National Institutes of Health grants P01 CA 116676 (to H.F.), People Program (Marie Curie Actions) of the European Union's Seventh Framework Program FP7/2007-2013/ under REA grant agreement No. PIRSES-GA-2013-612589: CHETCH (China and Europe taking care of healthcare solutions) (to X.Z.), and the National Natural Science Foundation of China (81503374) (to M.C.).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Oncology
  • Jin formula
  • Cisplatin
  • NSCLC
  • Apoptosis
  • PI3K/AKT pathway
  • CELL LUNG-CANCER
  • PTEN EXPRESSION
  • POOR-PROGNOSIS
  • APOPTOSIS
  • GROWTH
  • SENESCENCE
  • ARREST
  • KINASE
  • MTOR
  • EGFR

Study on Inhibitory Effect of MaiMenDong Decoction and WeiJing Decoction Combination with Cisplatin on NCI-A549 Xenograft in Nude Mice and Its Mechanism

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Journal Title:

Cancer Biology and Research

Volume:

Volume 8, Number 13

Publisher:

, Pages 2449-2455

Type of Work:

Article | Final Publisher PDF

Abstract:

MaiMenDong Decoction and WeiJing Decoction (Jin formula) is a traditional Chinese medication that consists of 8 medicinal plants, which recorded in the classical TCM literature Jin Kui Yao Lue and has been utilized in the treatment of lung diseases for hundreds of years in China. The present study aimed to determine the anti-tumor activity and the underlying mechanisms of Jin formula combined with cisplatin in the treatment of non-small cell lung cancer (NSCLC). Xenograft model of NCI-A549 was established in Balb/c nude mice. Five groups, including normal, MOCK, Jin, cisplatin (DDP), and Jin+DDP were included in the study. We found that Jin formula ameliorated the body weight loss caused by DDP 15 days after drug administration. Moreover, the combination of Jin with DDP enhanced the anti-tumor function of DDP. Microarray analysis showed that Jin suppressed gene expression of certain pathways which regulating cell cycle and apoptosis. Furthermore, DDP mainly decreased the gene expression level of angiogenesis associated factors, such as VEGFA, TGF-β and MMP-1. Moreover, co-treatment with Jin and DDP not only down-regulated Bcl-2 and E2F1, but also decreased the expression of MYC, MET, and MCAM. In addition, co-formula decreased the levels of p-AKT (thr308) and p-PTEN, increased Bax/Bcl-2 value, and resulted in apoptosis of tumor cells. Taken together, Jin+DDP significantly inhibited the growth of A549 cell transplanted solid tumor with slight side effect compared to the treatment by DDP only, and had a better effect than the Jin group. The mechanisms may be mainly associated with inactivation of PI3K/AKT pathway and apoptosis induction.

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