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Author Notes:

Author for Correspondence: Stephen Barnes, PhD, Department of Pharmacology and Toxicology, MCLM 452, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, Tel #: 205 934-7117; Fax #: 205 934-6944; sbarnes@uab.edu

We are indebted to faculty who have also previously contributed to the development of the workshop: Dr. Kathleen Stringer (University of Michigan), Dr. Dean P. Jones (Emory University), Dr. Grier P. Page (RTI International), Dr. Olga Ilkayeva (Duke University), Dr. Nikolaos Psychogios (Shire Pharmaceuticals) and Dr. Natalie Serkova (University of Colorado-Denver) and to faculty in receipt of NIH Administrative Supplements (Dr. Lalita Shevde-Sumant, R01 CA138850 and Dr. Adam Wende R00 HL111322).

We are also grateful for unrestricted support from Metabolon, Sciex and Waters. Acknowledgements are also given to Dr. N. Rama Krishna for the use of the Central Alabama NMR facility and to staff who assisted in the running of the workshop (UAB: Jennifer Spears, Lynn Waddell, Mikako Kawai, D. Ray Moore II, Landon Wilson, Ali Arabshahi, and Ronald Shin; RTI International, Rodney Snyder) and student trainees, Haley Albright, Kelly Walters and Sean Wilkinson.

Finally, the workshop has been enhanced by several plenary speakers: Dr. Stanley Hazen (Cleveland Clinic), Dr. Keith Baggerly (MD-Anderson), Dr. Richard Caprioli (Vanderbilt University), Dr. Arthur Edison (University of Florida) and Dr. David Wishart (University of Alberta), as well as speakers from several companies: Brigitte Simons and Jeremiah Tipton (SCIEX), Tom Beaty and John Shockcor (Waters) and Edward Karoly and Rob Mohney (Metabolon).

Subjects:

Research Funding:

Funds for the National Workshop in Metabolomics came from NIH grant R25 GM103798-03.

The workshop also received support from the UAB Office of the Vice-President for Research and Economic Development, the UAB School of Medicine and College of Arts and Science, the UAB Comprehensive Cancer Center (P30 CA13148), the UAB Comprehensive Diabetes Center, the UAB Diabetes Research Center (P60 DK079626), the UAB Center for Free Radical Biology, and the UAB-UCSD O’Brien Center for Acute Kidney Disease (P30 DK079337), and the Departments of Chemistry and Pharmacology and Toxicology.

Keywords:

  • data pre-processing
  • imaging metabolomics
  • isotopic labeling
  • multivariate analysis
  • pathway
  • univariate analysis

Training in metabolomics research. II. Processing and statistical analysis of metabolomics data, metabolite identification, pathway analysis, applications of metabolomics and its future

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Journal Title:

Journal of Mass Spectrometry

Volume:

Volume 51, Number 8

Publisher:

, Pages 535-548

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Metabolomics, a systems biology discipline representing analysis of known and unknown pathways of metabolism, has grown tremendously over the past 20 years. Because of its comprehensive nature, metabolomics requires careful consideration of the question(s) being asked, the scale needed to answer the question(s), collection and storage of the sample specimens, methods for extraction of the metabolites from biological matrices, the analytical method(s) to be employed and the quality control of the analyses, how collected data are correlated, the statistical methods to determine metabolites undergoing significant change, putative identification of metabolites and the use of stable isotopes to aid in verifying metabolite identity and establishing pathway connections and fluxes. This second part of a comprehensive description of the methods of metabolomics focuses on data analysis, emerging methods in metabolomics and the future of this discipline.

Copyright information:

Copyright © 2016 John Wiley & Sons, Ltd.

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