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Author Notes:

S. Hata is on leave from the National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba, Ibaraki 305, Japan.

The authors would like to express their gratitude to Drs. Dominique Blanchard, Hans Yssel, and Maria Grazia Roncarolo for culturing the T cell clones used in this study; Dr. William Strauss for providing the Vy3 and Vy4 probes ; and Drs. Michael Brenner, Michael Miller, Steven Porcelli, and Hamid Band for their comments on the manuscript.

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Research Funding:

This work was supported by grant DCB-8617540 from the National Science Foundation.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Immunology
  • Medicine, Research & Experimental
  • Research & Experimental Medicine
  • IMMUNOLOGY
  • MEDICINE, RESEARCH & EXPERIMENTAL

Diversity And Organization Of Human T-Cell Receptor-Delta Variable Gene Segments

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Journal Title:

Journal of Experimental Medicine

Volume:

Volume 169, Number 1

Publisher:

, Pages 41-57

Type of Work:

Article | Final Publisher PDF

Abstract:

Previous studies of the human TCR-δ gene identified a single commonly used V(δ) segment, denoted V(δ)1. To better understand the extent of the human TCR-δ V gene repertoire, TCR-δ transcripts and gene rearrangements were examined in a new panel of cloned human TCR-γ/δ lymphocytes. Through this analysis we identified and determined the structures of two new V(δ) segments, denoted V(δ)2 and V(δ)3. These V(δ) segments are different from previously characterized V(α) segments, supporting the notion that the human V(δ) and V(α) repertoires are distinct. Examination of V(γ) gene segment usage in these cells reveals that the V(δ)2 gene segment is used in conjunction with the V(γ)2 gene segment. Blot hybridization indicates that the V(δ)2 gene segment lies between V(δ)1 and D(δ)-J(δ)-C(δ), and within 100 kb of the latter. Analysis of genomic clones indicates that the V(δ)3 gene segment lies in an inverted orientation, ~2 kb 3' of C(δ). This implies that rearrangement of V(δ)3 to D(δ)-J(δ)-C(δ) occurs by inversion. Together with previous mapping studies, these results indicate that human V(δ) segments are dispersed, rather than clustered, within the TCR-α/δ locus. The analysis of rearrangements in polyclonal thymocyte DNA suggests that there may be a limited number of additional V(δ) gene segments yet to be characterized.

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m The Rockefeller University Press

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