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Author Notes:

Correspondence Address correspondence to: Michele A. Battle, mbattle@mcw.edu

For full list of acknowledgements, see final published version

The authors disclose no conflicts.

Subjects:

Research Funding:

Supported by the US National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases (DK087873 to M.A.B. and DK047987 to P.D.), Advancing a Healthier Wisconsin (M.A.B.), Hyundai Hope on Wheels Award (S.R.), and the Midwest Athletes against Childhood Cancer fund (S.R.).

Keywords:

  • Cyp7a1, cytochrome P450 family 7 subfamily A member 1
  • E, embryonic day
  • EMSA, electrophoretic mobility shift assay
  • Enterohepatic Signaling
  • FXR
  • FXR, farnesoid X receptor
  • Fabp6, fatty acid binding protein 6
  • Fgf, fibroblast growth factor
  • Fgf15
  • Jejunal Identity
  • OSTα/β, organic solute transporter α/β
  • PCR, polymerase chain reaction
  • SBS, short-bowel syndrome
  • Slc, solute carrier
  • TSS, transcription start site
  • Transcriptional Regulation
  • bio-ChIP-seq, biotin-mediated chromatin immunoprecipitation with high-throughput sequencing
  • bp, base pair
  • cDNA, complementary DNA
  • cKI, conditional knock-in
  • cKO, conditional knockout
  • dATP, deoxyadenosine triphosphate
  • lnl, loxP-flanked PGK-Neo-3xSV40 polyadenylation sequence
  • mRNA, messenger RNA
  • pA, polyadenylation
  • qRT, quantitative reverse-transcription
  • xiFABP, Xenopus I-FABP

GATA4 Is Sufficient to Establish Jejunal Versus Ileal Identity in the Small Intestine.

Journal Title:

Cellular and Molecular Gastroenterology and Hepatology

Volume:

Volume 3, Number 3

Publisher:

, Pages 422-446

Type of Work:

Article | Final Publisher PDF

Abstract:

BACKGROUND & AIMS: Patterning of the small intestinal epithelium along its cephalocaudal axis establishes three functionally distinct regions: duodenum, jejunum, and ileum. Efficient nutrient assimilation and growth depend on the proper spatial patterning of specialized digestive and absorptive functions performed by duodenal, jejunal, and ileal enterocytes. When enterocyte function is disrupted by disease or injury, intestinal failure can occur. One approach to alleviate intestinal failure would be to restore lost enterocyte functions. The molecular mechanisms determining regionally defined enterocyte functions, however, are poorly delineated. We previously showed that GATA binding protein 4 (GATA4) is essential to define jejunal enterocytes. The goal of this study was to test the hypothesis that GATA4 is sufficient to confer jejunal identity within the intestinal epithelium. METHODS: To test this hypothesis, we generated a novel Gata4 conditional knock-in mouse line and expressed GATA4 in the ileum, where it is absent. RESULTS: We found that GATA4-expressing ileum lost ileal identity. The global gene expression profile of GATA4-expressing ileal epithelium aligned more closely with jejunum and duodenum rather than ileum. Focusing on jejunal vs ileal identity, we defined sets of jejunal and ileal genes likely to be regulated directly by GATA4 to suppress ileal identity and promote jejunal identity. Furthermore, our study implicates GATA4 as a transcriptional repressor of fibroblast growth factor 15 (Fgf15), which encodes an enterokine that has been implicated in an increasing number of human diseases. CONCLUSIONS: Overall, this study refines our understanding of an important GATA4-dependent molecular mechanism to pattern the intestinal epithelium along its cephalocaudal axis by elaborating on GATA4's function as a crucial dominant molecular determinant of jejunal enterocyte identity. Microarray data from this study have been deposited into NCBI Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo) and are accessible through GEO series accession number GSE75870.

Copyright information:

© 2017 The Authors

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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