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Author Notes:

Correspondence: Elena Gómez-Díaz, Department of Biology, O. Wayne Rollins Research Center, Emory University, Room 1071B, 1510 Clifton Road NE, Atlanta, GA 30322, USA e-mail: elegomezdiaz@gmail.com

We thank The Genomic Services Lab at the HudsonAlpha Institute for Biotechnology for their help in preparing RNA-seq libraries and performing Illumina sequencing of RNA-seq and ChIP-Seq samples.

We thank A. Nicot, M. N. Lacroix, for assistance with mosquito breeding, A. Kotlar and K. Van Bortle for advice in the data analysis.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.


Research Funding:

This work was supported by U.S. Public Health Service Award R02GM035463 from the National Institutes of Health.


  • H3K27ac
  • H3K27me3
  • histone post-translational modifications
  • chromatin immunoprecipitation
  • gene expression regulation
  • mosquito-borne diseases
  • ChIP-seq
  • RNA-Seq

Insights into the epigenomic landscape of the human malaria vector Anopheles gambiae

Journal Title:

Frontiers in Genetics


Volume 5


Type of Work:

Article | Final Publisher PDF


The epigenome of the human malaria vector Anopheles gambiae was characterized in midgut cells by mapping the distribution and levels of two post-translational histone modifications, H3K27ac and H3K27me3. These histone profiles were then correlated with levels of gene expression obtained by RNA-seq. Analysis of the transcriptome of A. gambiae midguts and salivary glands led to the discovery of 13,898 new transcripts not present in the most recent genome assembly. A subset of these transcripts is differentially expressed between midgut and salivary glands. The enrichment profiles of H3K27ac and H3K27me3 are mutually exclusive and associate with high and low levels of transcription, respectively. This distribution agrees with previous findings in Drosophila showing association of these two histone modifications with either active or inactive transcriptional states, including Polycomb-associated domains in silenced genes. This study provides a mosquito epigenomics platform for future comparative studies in other mosquito species, opening future investigations into the role of epigenetic processes in vector-borne systems of medical and economic importance.

Copyright information:

© 2014 Gómez-Díaz, Rivero, Chandre and Corces.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/).

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