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Author Notes:

Address correspondence to G.M. Buck Louis, Division of Intramural Population Health Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Rockville, MD 20852 USA. Telephone: (301) 496-6155. E-mail: louisg@mail.nih.gov

We acknowledge the technical assistance of A. Calafat, Division of Laboratory Sciences, Centers for Disease Control and Prevention (CDC), who performed the analytic chemistry work; and the Reproductive Health Assessment Team, Biomonitoring and Health Assessment Branch, CDC, for the analysis of semen samples under Memorandums of Understanding with the Eunice Kennedy Shriver National Institute of Child Health and Human Development.

The authors declare they have no actual or potential competing financial interests.


Research Funding:

Supported by the Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development (N01-HD-3-3355, N01-HD-3-3356, and NOH-HD-3-3358).


  • Science & Technology
  • Life Sciences & Biomedicine
  • Environmental Sciences
  • Public, Environmental & Occupational Health
  • Toxicology
  • Environmental Sciences & Ecology
  • MEN
  • PFOS

Perfluorochemicals and Human Semen Quality: The LIFE Study


Journal Title:

Environmental Health Perspectives


Volume 123, Number 1


, Pages 57-63

Type of Work:

Article | Final Publisher PDF


BACKGROUND: The relation between persistent environmental chemicals and semen quality is evolving, although limited data exist for men recruited from general populations. OBJECTIVES: We examined the relation between perfluorinated chemicals (PFCs) and semen quality among 501 male partners of couples planning pregnancy. METHODS: Using population-based sampling strategies, we recruited 501 couples discontinuing contraception from two U.S. geographic regions from 2005 through 2009. Baseline interviews and anthropometric assessments were conducted, followed by blood collection for the quantification of seven serum PFCs (perfluorosulfonates, perfluorocarboxylates, and perfluorosulfonamides) using tandem mass spectrometry. Men collected a baseline semen sample and another approximately 1 month later. Semen samples were shipped with freezer packs, and analyses were performed on the day after collection. We used linear regression to estimate the difference in each semen parameter associated with a one unit increase in the natural log-transformed PFC concentration after adjusting for confounders and modeling repeated semen samples. Sensitivity analyses included optimal Box-Cox transformation of semen quality end points. RESULTS: Six PFCs [2-(N-methyl-perfluorooctane sulfonamido) acetate (Me-PFOSA-AcOH), perfluorodecanoate (PFDeA), perfluorononanoate (PFNA), perfluorooctane sulfonamide (PFOSA), perfluorooctane sulfonate (PFOS), and perfluorooctanoic acid (PFOA)] were associated with 17 semen quality end points before Box-Cox transformation. PFOSA was associated with smaller sperm head area and perimeter, a lower percentage of DNA stainability, and a higher percentage of bicephalic and immature sperm. PFDeA, PFNA, PFOA, and PFOS were associated with a lower percentage of sperm with coiled tails. CONCLUSIONS: Select PFCs were associated with certain semen end points, with the most significant associations observed for PFOSA but with results in varying directions.

Copyright information:

Publication of EHP lies in the public domain and is therefore without copyright.

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