About this item:

507 Views | 938 Downloads

Author Notes:

Corresponding Author: Kedibone M. Ndlangisa E-mail: kedibonen@nicd.ac.za

Conceived and designed the experiments: AVG KPK MDP NW.

Performed the experiments: KMN LDG.

Analyzed the data: KMN LDG MDP.

Contributed reagents/materials/analysis tools: AVG MDP.

Contributed to the writing of the manuscript: KMN LDG AVG KPK MDP NW.

We thank GERMS-SA (Group for Enteric, Respiratory and Meningeal Surveillance) for coordinating national surveillance of IPD in South Africa.

We are grateful to CRDM laboratory staff for their technical input and all clinical and laboratory staff throughout South Africa for submitting case reports and isolates for the GERMS-SA national surveillance program, and all patients whose isolates were used in the study.

We acknowledge the use of the pneumococcal MLST database which is located at Imperial College London and is funded by the Wellcome Trust.

Molecular characterization of isolates was funded by Pfizer Vaccines Research.

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Author Disclosures:Anne von Gottberg and Mignon du Plessis have received research funding from Pfizer.

In addition, Anne von Gottberg has received research funding from Sanofi Pasteur. Keith P. Klugman has received consultancy fees from Pfizer, Sanofi Pasteur, and Novartis.

The contents are solely the responsibility of the authors and do not necessarily represent the official views of the CDC

Subjects:

Research Funding:

This work was funded by the National Health Laboratory Service, Centers for Disease Control and Prevention (CDC), National Centre for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP), Global AIDS Program Cooperative Agreement U62/PSO022901.

Keywords:

  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics
  • SEROTYPE 19A
  • UNITED-STATES
  • GENETIC-STRUCTURE
  • TEMPORAL TRENDS
  • CHILDREN
  • CLONES
  • EPIDEMIOLOGY
  • VACCINATION
  • MENINGITIS
  • INFECTIONS

Population Snapshot of Streptococcus pneumoniae Causing Invasive Disease in South Africa Prior to Introduction of Pneumococcal Conjugate Vaccines

Tools:

Journal Title:

PLoS ONE

Volume:

Volume 9, Number 9

Publisher:

, Pages e107666-e107666

Type of Work:

Article | Final Publisher PDF

Abstract:

We determined the sequence types of isolates that caused invasive pneumococcal disease (IPD) prior to routine use of pneumococcal conjugate vaccines (PCV) in South Africa. PCV-13 serotypes and 6C isolates collected in 2007 (1 461/2 437, 60%) from patients of all ages as part of on-going, national, laboratory-based surveillance for IPD, were selected for genetic characterization. In addition, all 134 non-PCV isolates from children <2 years were selected for characterization. Sequence type diversity by serotype and age category (children <5 years vs. individuals ≥5 years) was assessed for PCV serotypes using Simpson's index of diversity. Similar genotypes circulated among isolates from children and adults and the majority of serotypes were heterogeneous. While globally disseminated clones were common among some serotypes (e.g., serotype 1 [clonal complex (CC) 217, 98% of all serotype 1] and 14 [CC230, 43%)]), some were represented mainly by clonal complexes rarely reported elsewhere (e.g., serotype 3 [CC458, 60%] and 19A [CC2062, 83%]). In children <2 years, serotype 15B and 8 were the most common serotypes among non-PCV isolates (16% [22/134] and 15% [20/134] isolates, respectively). Sequence type 7052 and 53 were most common among serotypes 15B and 8 isolates and accounted for 58% (7/12) and 64% (9/14) of the isolates, respectively. Serotype 19F, 14, 19A and 15B had the highest proportions of penicillin non-susceptible isolates. Genotypes rarely reported in other parts of the world but common among some of our serotypes highlight the importance of our data as these genotypes may emerge post PCV introduction. Copyright:

Copyright information:

© 2014 Ndlangisa et al.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
Export to EndNote