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Author Notes:

Address all correspondence to: Robert E. Gross, E-mail: rgross@emory.edu

Subjects:

Research Funding:

Funding sources: NS079268 and NS079757 to R.E.G.; NS086433 to J.K.T.; NSF 1512826 to K.B.; Duke Institute for Brain Science (DIBS Research Incubator Award) and MH101525 to U.H.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Physical Sciences
  • Neurosciences
  • Optics
  • Neurosciences & Neurology
  • bioluminescence
  • luciferase
  • imaging
  • live cell
  • in vivo
  • GREEN FLUORESCENT PROTEIN
  • RESONANCE ENERGY-TRANSFER
  • NEURAL STEM-CELLS
  • IN-VIVO
  • LUMINESCENT PROTEINS
  • LIVING SUBJECTS
  • SINGLE-CELL
  • REPORTER
  • BRAIN
  • LUCIFERASES

Bioluminescence imaging in live cells and animals

Tools:

Journal Title:

Neurophotonics

Volume:

Volume 3, Number 2

Publisher:

, Pages 025001-025001

Type of Work:

Article | Final Publisher PDF

Abstract:

The use of bioluminescent reporters in neuroscience research continues to grow at a rapid pace as their applications and unique advantages over conventional fluorescent reporters become more appreciated. Here, we describe practical methods and principles for detecting and imaging bioluminescence from live cells and animals. We systematically tested various components of our conventional fluorescence microscope to optimize it for long-term bioluminescence imaging. High-resolution bioluminescence images from live neurons were obtained with our microscope setup, which could be continuously captured for several hours with no signs of phototoxicity. Bioluminescence from the mouse brain was also imaged noninvasively through the intact skull with a conventional luminescence imager. These methods demonstrate how bioluminescence can be routinely detected and measured from live cells and animals in a cost-effective way with common reagents and equipment.

Copyright information:

© 2016 The Authors.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/).

Creative Commons License

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