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Author Notes:

Correspondence should be addressed to: KatalinTóth, Ph.D., Quebec Mental Health Institute, Department of Psychiatry and Neuroscience, Faculty of Medicine, Université Laval, 2601 chemin de la Canardière, Quebec City, QC, G1J 2G3 Canada, Tel: 418-663-5747/4702, Fax: 418-663-8756, katalin.toth@fmed.ulaval.ca.

A.E. V.F. and K.T. designed and planned the experiments, which were conducted by A.E. S.C. and K.T. KO animals were originally generated in the laboratory of V.F.

Data analysis was done by A.E., S.C. and K.T.

The paper was written by A.E., S.C., V.F. and K.T.

The authors thank Dr. GyörgyBuzsáki (NYU, USA) for generously providing the in vivo granule cell firing patterns for this study, and Dr. JaideepBains (University of Calgary, Canada) for helpful discussions and his thoughtful comments on the manuscript.

We thank Mr. Philippe Lemieux and Mr. Modesto R. Peralta III for their expert technical assistance.

The authors declare no competing financial interests.

Subjects:

Research Funding:

This work was supported by CIHR Operating grant to K.T. (MOP-81142) and grants from the National Institutes of Health (GM077569 and NS42599) and CHOA Children’s Center for Neuroscience to VF.

A.E was supported by CTRN fellowship and S.C. was supported by NSERC and CTRN scholarships.

Keywords:

  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics
  • MOSSY-FIBER SYNAPSES
  • SHORT-TERM PLASTICITY
  • SPONTANEOUS NEUROTRANSMITTER RELEASE
  • TRANSMITTER RELEASE
  • SYNAPTIC VESICLES
  • CA2+ SENSOR
  • GENETIC-ANALYSIS
  • NETWORK ACTIVITY
  • GRANULE CELLS
  • GABA RELEASE

Vesicles derived via AP-3-dependent recycling contribute to asynchronous release and influence information transfer

Tools:

Journal Title:

Nature Communications

Volume:

Volume 5

Publisher:

, Pages 5530-5530

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Action potentials trigger synchronous and asynchronous neurotransmitter release. Temporal properties of both types of release could be altered in an activity-dependent manner. While the effects of activity-dependent changes in synchronous release on postsynaptic signal integration have been studied, the contribution of asynchronous release to information transfer during natural stimulus patterns is unknown. Here we find that during trains of stimulations, asynchronous release contributes to the precision of action potential firing. Our data show that this form of release is selectively diminished in AP-3b2 KO animals, which lack functional neuronal AP-3, an adaptor protein regulating vesicle formation from endosomes generated during bulk endocytosis. We find that in the absence of neuronal AP-3, asynchronous release is attenuated and the activity-dependent increase in the precision of action potential timing is compromised. Lack of asynchronous release decreases the capacity of synaptic information transfer and renders synaptic communication less reliable in response to natural stimulus patterns.

Copyright information:

© 2014 Macmillan Publishers Limited. All rights reserved.

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