448 Views | 1,016 Downloads
Corresponding Authors: Monika Zieonka: Dept. of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226., Tel.: 414-955-4000; Fax: 414- 955-6512; E-mail: jkielonk@mcw.edu
Corresponding Authors: Balaraman Kalyanaraman:Dept. of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226., Tel.: 414-955-4000; Fax: 414- 955-6512; E-mail: balarama@mcw.edu
B. K. conceived and coordinated the study and wrote the paper.
J. Z. designed, performed, analyzed, and interpreted the data, prepared the figures, and revised the paper critically.
M. Z. performed the experiments and provided the technical assistance.
G. C. designed and performed the Seahorse experiments and obtained cell proliferation data.
M. H. and M. M. A. synthesized hydropropidine and cyclo-o-MitoPh product, respectively.
O. O. provided assistance in these syntheses and structure analyses.
R. P. synthesized o-MitoPhB(OH)2, its oxidation/nitration products and corresponding deuterated analogs.
A. S. helped in characterization of minor products of o-MitoPhB(OH)2 oxidation.
J. D. L. revised the manuscript for intellectual content.
L. V. provided training, assisted in screening of the bioactive libraries at Broad Institute, and performed first pass analysis of the screening results.
All authors reviewed the results and approved the final version of the manuscript.
LC-MS/MS analyses were performed at the Cancer Center Bioenergetics Shared Resource at the Medical College of Wisconsin.
The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
The authors declare that they have no conflicts of interest with the contents of this article.
This work was supported by National Institutes of Health Grant R01 HL073056 (to B. K.) and in part by Molecular Libraries Probe Development Network Grant U54 HG005032.
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.