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Correspondence and requests for materials should be addressed to G.K. (email: george.kassiotis@crick.ac.uk).
J.M. performed and interpreted experiments and wrote the manuscript.
M.J.P., U.E., G.T. and A.F. performed and interpreted experiments.
R.A. and B.E. designed, performed and interpreted experiments relating to two-dimensional TCR affinity measurements.
M.P. designed, prepared and provided the MHC II tetramer used in this study.
G.K. designed and interpreted the experiments, wrote the manuscript and supervised the study.
We wish to thank Dr Kristin A. Hogquist for the Nur77-GFP mice, Dr Toshitada Takemori for the conditional Bcl6 mice, Dr Kim Hasenkrug for FV and FBL-3 stocks, Dr Ulf Dittmer for the Ad5.pIX-gp70 stocks and Drs Anne O'Garra, Brigitta Stockinger, Mark Wilson and Eva Frickel for helpful comments and discussion. We are grateful for assistance from the Biological Services, Flow Cytometry and Protein Purification Facilities at the Francis Crick institute.
Accession codes: The Sequence Read Archive (SRA) and European Nucleotide Archive (ENA) accession numbers for the TCR sequences reported in this paper are SRX750575 and PRJEB11747, respectively.
This work was supported by the Francis Crick Institute which receives its core funding from Cancer Research UK, the UK Medical Research Council (U117581330 to G.K.), and the Wellcome Trust.
© 2016, Nature Publishing Group.