The Drosophila ortholog of the Zc3h14 RNA binding protein acts within neurons to pattern axon projection in the developing brain

• Authors: Seth Kelly, Emory University; Rick Bienkowski, Emory University; Ayan Banerjee, Emory University; David J. Melicharek, Drexel University; Zachariah A. Brewer, College of Wooster; Daniel R. Marenda, Drexel University; Anita Corbett, Emory University; Kenneth Moberg, Emory University
• Journal Title: Developmental Neurobiology
• Volume: Volume 76, Number 1
• Publisher: Wiley-Blackwell | 2016-01-01, Pages 93-106
• Type of Work: Article | Post-print: After Peer Review
• Publisher DOI: http://doi.org/10.1002/dneu.22301
• Subjects: Biology, Genetics; Biology, Cell
• Keywords: Science & Technology; Life Sciences & Biomedicine; Developmental Biology; Neurosciences; Neurosciences & Neurology; Drosophila; mushroom body development; polyadenosine RNA binding protein; dNab2; MENTAL-RETARDATION PROTEIN; MUSHROOM BODY DEVELOPMENT; REPRESSIBLE CELL MARKER; POLYADENOSINE RNA; MOSAIC ANALYSIS; BODIES; GUIDANCE; DSCAM; MORPHOGENESIS; NEUROTOXICITY

Abstract

The dNab2 polyadenosine RNA binding protein is the D. melanogaster ortholog of the vertebrate ZC3H14 protein, which is lost in a form of inherited intellectual disability (ID). Human ZC3H14 can rescue D. melanogaster dNab2 mutant phenotypes when expressed in all neurons of the developing nervous system, suggesting that dNab2/ZC3H14 performs well-conserved roles in neurons. However, the cellular and molecular requirements for dNab2/ZC3H14 in the developing nervous system have not been defined in any organism. Here we show that dNab2 is autonomously required within neurons to pattern axon projection from Kenyon neurons into the mushroom bodies, which are required for associative olfactory learning and memory in insects. Mushroom body axons lacking dNab2 project aberrantly across the brain midline and also show evidence of defective branching. Coupled with the prior finding that ZC3H14 is highly expressed in rodent hippocampal neurons, this requirement for dNab2 in mushroom body neurons suggests that dNab2/ZC3H14 has a conserved role in supporting axon projection and branching. Consistent with this idea, loss of dNab2 impairs short-term memory in a courtship conditioning assay. Taken together these results reveal a cell-autonomous requirement for the dNab2 RNA binding protein in mushroom body development and provide a window into potential neurodevelopmental functions of the human ZC3H14 protein.