About this item:

398 Views | 846 Downloads

Author Notes:

Correspondence: Shonna M. McBride, shonna.mcbride@emory.edu.

AE, SK, RP, LJ, SA, and SM contributed to the acquisition and analysis of the data.

AE drafted the manuscript and AE, SK, RP, LJ, SA, and SM edited and approved the content.

We thank the members of the McBride lab for helpful suggestions and discussions throughout the course of this study. We are grateful to Brendan Wren for the gift of the R20291 spo0A mutant and to Aimee Shen for the spo0A plasmid.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The content of this manuscript is solely the responsibility of the authors and does not necessarily reflect the official views of the National Institutes of Health.

Subjects:

Research Funding:

This research was supported by the U.S. National Institutes of Health (NIH) through grants AI116933, AI109526, DK087763 and DK101870 to SM, AI106699 to SA, and GM099644 to RP.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Microbiology
  • Clostridium difficile
  • Clostridium difficile infection (CDI)
  • anaerobe
  • spores
  • resistance
  • germination
  • sporulation
  • BACILLUS-SUBTILIS
  • HYDROGEN-PEROXIDE
  • BACTERIAL-SPORES
  • EXOSPORIUM LAYER
  • INFECTION

Chemical and Stress Resistances of Clostridium difficile Spores and Vegetative Cells

Tools:

Journal Title:

Frontiers in Microbiology

Volume:

Volume 7, Number OCT

Publisher:

, Pages 1698-1698

Type of Work:

Article | Final Publisher PDF

Abstract:

Clostridium difficile is a Gram-positive, sporogenic and anaerobic bacterium that causes a potentially fatal colitis. C. difficile enters the body as dormant spores that germinate in the colon to form vegetative cells that secrete toxins and cause the symptoms of infection. During transit through the intestine, some vegetative cells transform into spores, which are more resistant to killing by environmental insults than the vegetative cells. Understanding the inherent resistance properties of the vegetative and spore forms of C. difficile is imperative for the development of methods to target and destroy the bacterium. The objective of this study was to define the chemical and environmental resistance properties of C. difficile vegetative cells and spores. We examined vegetative cell and spore tolerances of three C. difficile strains, including 630Δerm, a 012 ribotype and a derivative of a past epidemic strain; R20291, a 027 ribotype and current epidemic strain; and 5325, a clinical isolate that is a 078 ribotype. All isolates were tested for tolerance to ethanol, oxygen, hydrogen peroxide, butanol, chloroform, heat and sodium hypochlorite (household bleach). Our results indicate that 630Δerm vegetative cells (630 spo0A) are more resistant to oxidative stress than those of R20291 (R20291 spo0A) and 5325 (5325 spo0A). In addition, 5325 spo0A vegetative cells exhibited greater resistance to organic solvents. In contrast, 630Δerm spores were more sensitive than R20291 or 5325 spores to butanol. Spores from all three strains exhibited high levels of resistance to ethanol, hydrogen peroxide, chloroform and heat, although R20291 spores were more resistant to temperatures in the range of 60-75°C. Finally, household bleach served as the only chemical reagent tested that consistently reduced C. difficile vegetative cells and spores of all tested strains. These findings establish conditions that result in vegetative cell and spore elimination and illustrate the resistance of C. difficile to common decontamination methods. These results further demonstrate that the vegetative cells and spores of various C. difficile strains have different resistance properties that may impact decontamination of surfaces and hands.

Copyright information:

© 2016 Edwards, Karim, Pascual, Jowhar, Anderson and McBride.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
Export to EndNote