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Author Notes:

Corresponding Author: Joshua T. Maxwell Email: joshua.t.maxwell@emory.edu

Joshua T. Maxwell and Michael E. Davis were responsible for conception and design of the experiments.

Collection, analysis, and interpretation of data were carried out by Joshua T. Maxwell, Mary B. Wagner, and Michael E. Davis. Joshua T. Maxwell drafted the article.

All authors have approved the final version of the manuscript.

The authors wish to acknowledge the support of the Children's Miracle Network gift to Children's Healthcare of Atlanta.

The authors also wish to acknowledge the generous support of Ms. Katrina Ceccoli and the Darryll M. Ceccoli Research Fund, as well as Keilani and Brian Betkowski and the Betkowski Pediatric Research Fund.

Subjects:

Research Funding:

This work was also supported by federal funds from the National Heart, Lung, and Blood Institute HL088488 to Mary B. Wagner as well as American Heart Association Grant 11GRNT7490000 to Mary B. Wagner.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Cell & Tissue Engineering
  • Cell Biology
  • MESENCHYMAL STEM-CELLS
  • MYOCARDIAL REGENERATION
  • INFARCTED MYOCARDIUM
  • C-KIT(+) CELLS
  • HEART
  • DIFFERENTIATION
  • CARDIOMYOCYTES
  • TRANSPLANTATION
  • ISCHEMIA
  • SURVIVAL

Electrically Induced Calcium Handling in Cardiac Progenitor Cells

Tools:

Journal Title:

Stem Cells International

Volume:

Volume 2016

Publisher:

, Pages 8917380-8917380

Type of Work:

Article | Final Publisher PDF

Abstract:

For nearly a century, the heart was viewed as a terminally differentiated organ until the discovery of a resident population of cardiac stem cells known as cardiac progenitor cells (CPCs). It has been shown that the regenerative capacity of CPCs can be enhanced by ex vivo modification. Preconditioning CPCs could provide drastic improvements in cardiac structure and function; however, a systematic approach to determining a mechanistic basis for these modifications founded on the physiology of CPCs is lacking. We have identified a novel property of CPCs to respond to electrical stimulation by initiating intracellular Ca2+ oscillations. We used confocal microscopy and intracellular calcium imaging to determine the spatiotemporal properties of the Ca2+ signal and the key proteins involved in this process using pharmacological inhibition and confocal Ca2+ imaging. Our results provide valuable insights into mechanisms to enhance the therapeutic potential in stem cells and further our understanding of human CPC physiology.

Copyright information:

© 2016 Joshua T. Maxwell et al.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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