About this item:

205 Views | 743 Downloads

Author Notes:

Corresponding Author: Kerry J. Ressler Email: kressler@mclean.harvard.edu

This study was initiated and designed by K.M.M., D.C., D.G. R and K.J.R.

Funding was obtained by K.J.R. K.M.M. performed major behavioural experiments, viral infusions, pharmacological and optical manipulations.

J.G. and D.G.R performed and analysed electrophysiological experiments.

K.M.M and K.Z. performed tracing studies.

J.W. performed immunohistochemistry studies.

Primary writing was performed by K.M.M. and K.J.R., with edits from all authors.

We thank all the members of the Ressler Lab and Rainnie Lab for excellent insight and discussion of data.

Dr Jasnow for initiating experimental design. Abe Kim for excellent help in the lab with all things.

Torsten Klengel and Filomene Morrison for providing feedback.

Animal Care at YNPRC for all of their help.

Disclosure: The authors declare no competing financial interests.

Subjects:

Research Funding:

Support was provided by NIH (T32-GM08605, R01MH108665, and R01MH096764) and by an NIH/NCRR base grant (P51RR000165) to Yerkes National Primate Research Center.

Keywords:

  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics
  • TRANSGENIC MICE
  • NEUROTENSIN RECEPTORS
  • BETA-LACTOTENSIN
  • CONDITIONED FEAR
  • REMOTE-CONTROL
  • MECHANISMS
  • CIRCUITS
  • BRAIN
  • MEMORY
  • ADULT

Molecular characterization of Thy1 expressing fear-inhibiting neurons within the basolateral amygdala

Tools:

Journal Title:

Nature Communications

Volume:

Volume 7

Publisher:

, Pages 13149-13149

Type of Work:

Article | Final Publisher PDF

Abstract:

Molecular characterization of neuron populations, particularly those controlling threat responses, is essential for understanding the cellular basis of behaviour and identifying pharmacological agents acting selectively on fear-controlling circuitry. Here we demonstrate a comprehensive workflow for identification of pharmacologically tractable markers of behaviourally characterized cell populations. Thy1-eNpHR-, Thy1-Cre-and Thy1-eYFP-labelled neurons of the BLA consistently act as fear inhibiting or 'Fear-Off' neurons during behaviour. We use cell-type-specific optogenetics and chemogenetics (DREADDs) to modulate activity in this population during behaviour to block or enhance fear extinction. Dissociated Thy1-eYFP neurons are isolated using FACS. RNA sequencing identifies genes strongly upregulated in RNA of this population, including Ntsr2, Dkk3, Rspo2 and Wnt7a. Pharmacological manipulation of neurotensin receptor 2 confirms behavioural effects observed in optogenetic and chemogenetic experiments. These experiments identify and validate Ntsr2-expressing neurons within the BLA, as a putative 'Fear-Off' population.

Copyright information:

© 2016 The Author(s).

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
Export to EndNote