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Author Notes:

Email: biolrc@emory.edu

DK, Performed the experiments and analyzed the experimental data.

WHB, Wrote the programs and performed the computer simulations.

GSC, Supervised the project and provided valuable input.

RLC, Supervised the project and provided valuable input.

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

The other authors declare that no competing interests exist.

Subjects:

Research Funding:

National Institute of Neurological Disorders and Stroke R01 NS085006 to Ronald L Calabrese. National Science Foundation PHY-0750456 to Gennady S Cymbalyuk.

National Institute of Neurological Disorders and Stroke R01 NS085006 to Ronald L Calabrese.

National Science Foundation PHY-0750456 to Gennady S Cymbalyuk.

Na + /K + pump interacts with the h -current to control bursting activity in central pattern generator neurons of leeches

Tools:

Journal Title:

eLife

Volume:

Volume 5

Publisher:

, Pages e19322- e19322

Type of Work:

Article | Final Publisher PDF

Abstract:

The dynamics of different ionic currents shape the bursting activity of neurons and networks that control motor output. Despite being ubiquitous in all animal cells, the contribution of the Na+/K+ pump current to such bursting activity has not been well studied. We used monensin, a Na+/H+ antiporter, to examine the role of the pump on the bursting activity of oscillator heart interneurons in leeches. When we stimulated the pump with monensin, the period of these neurons decreased significantly, an effect that was prevented or reversed when the h-current was blocked by Cs+. The decreased period could also occur if the pump was inhibited with strophanthidin or K+-free saline. Our monensin results were reproduced in model, which explains the pump’s contributions to bursting activity based on Na+ dynamics. Our results indicate that a dynamically oscillating pump current that interacts with the h-current can regulate the bursting activity of neurons and networks.

Copyright information:

© 2016, Kueh et al

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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