About this item:

970 Views | 1,125 Downloads

Author Notes:

To whom correspondence should be addressed. Tel: +1 404 727 3729; Fax: +1 404 727 5408; Email: peng.jin@emory.edu Correspondence may also be addressed to Hongjun Song. Tel: +1 443 287 7499; Fax: +1 410 614 9568; Email: shongju1@jhmi.edu Correspondence may also be addressed to Hengli Tang. Tel: +1 850 645 2402; Fax: +1 850 645 8447; Email: tang@bio.fsu.edu Correspondence may also be addressed to Guo-li Ming. Tel: +1 443 287 7498; Fax: +1 410 614 9568; Email: gming1@jhmi.edu

We thank Dr Robert Tesh at UTMB and the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) for FSS 13025 isolate of ZIKV, Lihong Liu and Yuan Cai of the Ming and Song labs for technical assistance.

In addition, we would like to thanks C. Strauss for critical reading of the manuscript, Weining Tang at Omega Bioservices (Omega Bio-tek, Inc) and Mike Zwick and Ben Isett at the Emory Integrated Genomics Core (EIGC) for assistance with high-throughput sequencing.

The content is solely the responsibility of the authors and does not necessarily reflect the official views of the National Institutes of Health.

Conflict of interest statement. None declared.

Subjects:

Research Funding:

National Institutes of Health (NIH) [AI119530 and AI111250 to H.T., NS048271 and NS095348 to G-L.M., NS047344 and MH087874 to H.S., NS079625 to P.J.]; Maryland Stem Cell Research Fund (to H.S. and Z.W.); start-up fund (to H.S. and G-L.M.); College of Arts and Sciences and Department of Biological Science at Florida State University seed fund (to H.T.); Emory Genetics Discovery Fund (to P.J.).

This study was supported in part by the Emory Integrated Genomics Core (EIGC), which is subsidized by the Emory University School of Medicine and is one of the Emory Integrated Core Facilities.

Additional support was provided by the National Center for Advancing Translational Sciences of the National Institutes of Health under Award Number UL1TR000454.

Funding for open access charge: NIH.

Molecular signatures associated with ZIKV exposure in human cortical neural progenitors

Show all authors Show less authors

Journal Title:

Nucleic Acids Research

Volume:

Volume 44, Number 18

Publisher:

, Pages 8610-8620

Type of Work:

Article | Final Publisher PDF

Abstract:

Zika virus (ZIKV) infection causes microcephaly and has been linked to other brain abnormalities. How ZIKV impairs brain development and function is unclear. Here we systematically profiled transcriptomes of human neural progenitor cells exposed to Asian ZIKVC, African ZIKVM, and dengue virus (DENV). In contrast to the robust global transcriptome changes induced by DENV, ZIKV has a more selective and larger impact on expression of genes involved in DNA replication and repair. While overall expression profiles are similar, ZIKVC, but not ZIKVM, induces upregulation of viral response genes and TP53. P53 inhibitors can block the apoptosis induced by both ZIKVC and ZIKVM in hNPCs, with higher potency against ZIKVC-induced apoptosis. Our analyses reveal virus- and strain-specific molecular signatures associated with ZIKV infection. These datasets will help to investigate ZIKV-host interactions and identify neurovirulence determinants of ZIKV.

Copyright information:

© The Author(s) 2016.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/).

Creative Commons License

Export to EndNote