About this item:

221 Views | 478 Downloads

Author Notes:

Correspondence: Email: martin.moore@emory.edu; Tel.: +1-404-785-0977; Fax: +1-404-727-9223

C.A.R. and M.L.M. coauthored the manuscript.

M.C.C. performed fluorescent imaging shown in Figure 1.

Subjects:

Research Funding:

This work was supported by NIH grants 1R01AI087798, 1U19AI095227, 5K12HD072245, and by Emory University and Children’s Healthcare of Atlanta funds.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Virology
  • fluorescent reporter virus
  • bioluminescent reporter virus
  • paramyxovirus
  • orthomyxovirus
  • RESPIRATORY SYNCYTIAL VIRUS
  • INFLUENZA-A VIRUS
  • REVERSE GENETICS SYSTEM
  • POLARIZED EPITHELIAL-CELLS
  • ONCOLYTIC MEASLES-VIRUS
  • OPEN READING FRAME
  • RNA-POLYMERASE-II
  • IN-VIVO
  • LUCIFERASE REPORTER
  • MONOMERIC RED

Fluorescent and Bioluminescent Reporter Myxoviruses

Tools:

Journal Title:

Viruses

Volume:

Volume 8, Number 8

Publisher:

, Pages 214-214

Type of Work:

Article | Final Publisher PDF

Abstract:

The advent of virus reverse genetics has enabled the incorporation of genetically encoded reporter proteins into replication-competent viruses. These reporters include fluorescent proteins which have intrinsic chromophores that absorb light and re-emit it at lower wavelengths, and bioluminescent proteins which are luciferase enzymes that react with substrates to produce visible light. The incorporation of these reporters into replication-competent viruses has revolutionized our understanding of molecular virology and aspects of viral tropism and transmission. Reporter viruses have also enabled the development of high-throughput assays to screen antiviral compounds and antibodies and to perform neutralization assays. However, there remain technical challenges with the design of replication-competent reporter viruses, and each reporter has unique advantages and disadvantages for specific applications. This review describes currently available reporters, design strategies for incorporating reporters into replication-competent paramyxoviruses and orthomyxoviruses, and the variety of applications for which these tools can be utilized both in vitro and in vivo.

Copyright information:

© 2016 by the authors

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
Export to EndNote