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Author Notes:
Corresponding Author: Brian Evavold, bevavol@emory.edu, (404) 727-3393.
Aaron Rae at Emory+Children’s Pediatric Research Immunology Core carried out FACS. Benjamin Barwick created programs for sequence analysis. Laurel Lawrence maintained the mouse colony. The authors would like to thank Lori Blanchfield, Rakieb Andargarchew, Jennifer Cosby and Emily Cartwright for helpful discussion.
The authors have no conflicts of interest to disclose.
Subjects:
Research Funding:
This work was supported by NIH grant T32 AI007610, RO1 NS071518 and National Multiple Sclerosis Society grant RG4047.
Keywords:
- Science & Technology
- Life Sciences & Biomedicine
- Biochemical Research Methods
- Immunology
- Biochemistry & Molecular Biology
- T cell receptor
- Clonotype
- Lineage tracking
- NAIVE
- IMMUNOINFORMATICS
- DIFFERENTIATION
- POPULATIONS
- TOOLS
Identification of T cell clones without the need for sequencing
Abstract:
The brainbow recombination fluorescent protein system has been used for a multitude of applications in fate and lineage tracking. Here, we use a mouse with a ubiquitously expressed brainbow construct, termed the Confetti mouse, to perform T lymphocyte cell lineage tracking. We demonstrate that antigen-specific T lymphocyte clonotypes can be identified and phenotyped using flow cytometry instead of performing expensive and time-consuming methods of single cell sequencing.
Copyright information:
© 2015 Elsevier B.V.
This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).
