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Author Notes:

Corresponding author: Richard D. Cummings, Email:rdcummi@emory.edu

Subjects:

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Biochemical Research Methods
  • Biochemistry & Molecular Biology
  • Glycomics
  • Glycan microarrays
  • Influenza virus
  • Fluorescence detection
  • Glycan-binding motif
  • H3N2 INFLUENZA-VIRUSES
  • NEURAMINIDASE
  • SPECIFICITY
  • ARRAY

Probing Virus-Glycan Interactions Using Glycan Microarrays

Tools:

Journal Title:

Carbohydrate Microarrays

Volume:

Volume 808

Publisher:

, Pages 251-267

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Glycan microarrays are surfaces that contain immobilized oligosaccharides or glycoconjugates and have proven useful in probing the interactions between glycan-binding proteins (GBPs) and individual glycans. Such glycan microarrays have been especially important in studying virus-glycan interactions, as most viruses express one or more GBPs important for pathogenesis. For studying interactions of influenza viruses with glycans, we describe protocols for fluorescent labeling of virus, addition of virus to a glycan microarray, analysis of a glycan microarray slide experiment, and interpretation of data. © 2012 Springer Science+Business Media, LLC.

Copyright information:

© 2012, Springer Science+Business Media, LLC

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