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Author Notes:

Correspondence to:Sulma I. Mohammed, Email: mohammes@purdue.edu

All authors declare no conflicts of interest.

Subjects:

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Oncology
  • Cell Biology
  • cervical
  • cancer
  • LMIC
  • Africa
  • point-of-care
  • VALOSIN-CONTAINING PROTEIN
  • SUB-SAHARAN AFRICA
  • HUMAN-PAPILLOMAVIRUS
  • CLINICAL-PERFORMANCE
  • ELEVATED EXPRESSION
  • PATHOGEN DETECTION
  • GOLD NANOPARTICLE
  • P97
  • CARCINOMA
  • PROGNOSIS

Point-of-care test for cervical cancer in LMICs

Tools:

Journal Title:

Oncotarget

Volume:

Volume 7, Number 14

Publisher:

, Pages 18787-18797

Type of Work:

Article | Final Publisher PDF

Abstract:

Cervical cancer screening using Papanicolaou's smear test has been highly effective in reducing death from this disease. However, this test is unaffordable in low- and middle-income countries, and its complexity has limited wide-scale uptake. Alternative tests, such as visual inspection with acetic acid or Lugol's iodine and human papillomavirus DNA, are sub-optimal in terms of specificity and sensitivity, thus sensitive and affordable tests with high specificity for on-site reporting are needed. Using proteomics and bioinformatics, we have identified valosin-containing protein (VCP) as differentially expressed between normal specimens and those with cervical intra-epithelial neoplasia grade 2/3 (CIN2/CIN3+) or worse. VCP-specific immunohistochemical staining (validated by a point-of-care technology) provided sensitive (93%) and specific (88%) identification of CIN2/CIN3+ and may serve as a critical biomarker for cervical-cancer screening. Future efforts will focus on further refinements to enhance analytic sensitivity and specificity of our proposed test, as well as on prototype development.

Copyright information:

© 2016 Mohammed et al.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/).

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