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Author Notes:

Correspondence to: Department of Biology, Georgia State University, 161 Jesse Hill Jr. Dr, Atlanta, GA30303. Email: cgarnettbenson@gsu.edu

The authors thank Mr. Wen Huang and Dr. Jeff Chou for excellent technical assistance in the experiments described here.

Subjects:

Research Funding:

This work was supported by R01 CA127621 from the National Cancer Institute and by an award from the Children's Leukemia Research Association.

Microarray analysis was provided by the Cancer Genomics Shared Resource of the Comprehensive Cancer Center of Wake Forest University supported by NCI CCSG P30CA012197 grant.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Virology
  • Human adenovirus
  • Species C
  • Lymphocytes
  • Latent
  • Gene-expression
  • Leukemia
  • Oncogenesis
  • Viral persistence
  • Adenovirus
  • Human lymphocytes
  • EPSTEIN-BARR-VIRUS
  • LYMPHOBLASTIC-LEUKEMIA
  • EPIGENETIC REGULATION
  • COEXPRESSED GENES
  • AMNIOTIC-FLUID
  • CANCER-CELLS
  • B-CELLS
  • PROTEIN
  • DNA
  • BIOINFORMATICS

Limited but durable changes to cellular gene expression in a model of latent adenovirus infection are reflected in childhood leukemic cell lines

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Journal Title:

Virology

Volume:

Volume 494

Publisher:

, Pages 67-77

Type of Work:

Article | Final Publisher PDF

Abstract:

© 2016 The Authors.Mucosal lymphocytes support latent infections of species C adenoviruses. Because infected lymphocytes resist re-infection with adenovirus, we sought to identify changes in cellular gene expression that could inhibit the infectious process. The expression of over 30,000 genes was evaluated by microarray in persistently infected B-and T-lymphocytic cells. BBS9, BNIP3, BTG3, CXADR, SLFN11 and SPARCL1 were the only genes differentially expressed between mock and infected B cells. Most of these genes are associated with oncogenesis or cancer progression. Histone deacetylase and DNA methyltransferase inhibitors released the repression of some of these genes. Cellular and viral gene expression was compared among leukemic cell lines following adenovirus infection. Childhood leukemic B-cell lines resist adenovirus infection and also show reduced expression of CXADR and SPARCL. Thus adenovirus induces limited changes to infected B-cell lines that are similar to changes observed in childhood leukemic cell lines.

Copyright information:

© 2016 The Authors.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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