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Author Notes:

gsilves@emory.edu

Conceived and designed the experiments: AMO GS.

Performed the experiments: AMO DGC JY KMS.

Analyzed the data: AMO DGC PK AR MPD GS.

Contributed reagents/materials/analysis tools: THV NRK JMB MPD GS.

Wrote the paper: AMO GS.

The authors have declared that no competing interests exist.

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Research Funding:

This work was supported primarily by R37-AI066998 to GS (URL: www.grants.nih.gov). In addition, it was supported by the National Institutes of Health/National Center for Research Resources (P51RR000165) and is currently supported by the Office of Research Infrastructure Programs / OD P51OD011132 to the Yerkes National Primate Research Center.

Keywords:

  • Science & Technology
  • Multidisciplinary Sciences
  • SIMIAN IMMUNODEFICIENCY VIRUS
  • CD4(+) T-CELLS
  • LYMPHOCYTE TURNOVER
  • HIV-1 INFECTION
  • MACAQUES
  • SUBPOPULATIONS
  • DYNAMICS
  • RATES
  • T cells
  • SIV
  • Cytotoxic T cells
  • Bromodeoxyuridine labeling
  • Memory
  • Memory T cells
  • Cell death
  • Flow cytometry

Analysis of the In Vivo Turnover of CD4+T-Cell Subsets in Chronically SIV-Infected Sooty Mangabeys

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Journal Title:

PLoS ONE

Volume:

Volume 11, Number 5

Publisher:

, Pages e0156352-e0156352

Type of Work:

Article | Final Publisher PDF

Abstract:

Aberrant turnover of memory CD4+ T-cells is central to Acquired Immunodeficiency Syndrome (AIDS) progression. Understanding the relationship between the turnover of CD4+ subsets and immunological homeostasis during simian immunodeficiency virus (SIV) infection in natural hosts may provide insight into mechanisms of immune regulation that may serve as models for therapeutic intervention in Human Immunodeficiency Virus (HIV)-infected persons. Sooty mangabeys (SMs) have naturally evolved with SIV to avoid AIDS progression while maintaining healthy peripheral CD4+ T-cell counts and thus represent a model by which therapeutic interventions for AIDS progression might be elucidated. To assess the relationship between the turnover of CD4+ subsets and immunological homeostasis during SIV infection in non-progressive hosts, we treated 6 SIV-uninfected and 9 SIVinfected SMs with 2′-bromo-5′-deoxyuridine (BrdU) for 14 days and longitudinally assessed CD4+ T-cell subset turnover by polychromatic flow cytometry. We observed that, in SIV-infected SMs, turnover of CD4+ T-cell naïve and central, transitional, and effector memory subsets is comparable to that in uninfected animals. Comparable turnover of CD4+ T-cell subsets irrespective of SIV-infection status likely contributes to the lack of aberrant immune activation and disease progression observed after infection in non-progressive hosts.

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This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an Open Access work distributed under the terms of the Creative Commons Universal : Public Domain Dedication License (http://creativecommons.org/publicdomain/zero/1.0/).

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