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Author Notes:

To whom correspondence should be addressed: H. Ohno. Tel. +81 45-503-7031. Fax. +81 45-503-7030. E-mail: ohno@rcai.riken.jp

We thank Ms. Y. Yamada for secretarial assistance.

Subjects:

Research Funding:

This study was supported in part by Grants-in-Aid for Scientific Research (B) (H.O.), Young Scientists (K.H. and S.F.), Scientific Research on Innovative Areas (H.O.) and Scientific Research in Priority Areas (K.H.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and Takeda Science Foundation (K.H.).

Keywords:

  • Life Sciences & Biomedicine
  • Genetics & Heredity
  • M cell
  • the bursa of Fabricius
  • annexin A10
  • cellular prion protein
  • FOLLICULAR DENDRITIC CELLS
  • PATCH M-CELLS
  • PRION PROTEIN
  • EPITHELIAL-CELLS
  • PEYERS-PATCHES
  • EXPRESSION
  • ANNEXINS
  • MACROPHAGES
  • INVOLVEMENT
  • FABRICIUS

New Approach for M-Cell-Specific Molecules Screening by Comprehensive Transcriptome Analysis

Tools:

Journal Title:

DNA Research

Volume:

Volume 16, Number 4

Publisher:

, Pages 227-235

Type of Work:

Article | Final Publisher PDF

Abstract:

A minor population of M cells within the follicle-associated epithelium (FAE) of intestinal Peyer's patches (PPs) serves as a major portal for entry of exogenous antigens. Characterization of the mammalian M cells, including identification of M-cell surface molecules used for bacterial uptake, has been hampered by their relative rarity. In contrast, M cells constitute virtually all of the FAE cells in the avian bursa of Fabricius. We therefore performed comparative gene expression profiling of chicken and murine FAE to identify commonly expressed genes by M cells in both species. The comprehensive transcriptome analysis revealed that 28 genes were commonly up-regulated in FAE from both species. In situ hybridization revealed that annexin A10 (Anxa10) mRNA was scattered in FAE, and co-localized with Ulex europaeus agglutinin-1 binding to M cells. Whole-mount immunostaining also revealed that cellular prion protein (PrPC) was expressed on the luminal side of the apical plasma membrane of M cells, and co-localized with grycoprotein 2 that recognizes only M cells in murine PP. Our findings identify new M-cell-specific molecules through using comprehensive transcriptome analysis. These conserved molecules in M cells of mice and chickens may play essential roles in M-cell function and/or differentiation.

Copyright information:

© The Author 2009. Kazusa DNA Research Institute. The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org.

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