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Research Funding:
This work has been presented in part at the annual meeting for Association for Research in Vision and Ophthalmology, May 4, 2009, Fort Lauderdale, FL, supported in part by NIH NEI R24 EY017045, P30 EY06360, and an unrestricted departmental grant from Research to Prevent Blindness, Inc. The authors have no proprietary interests in the materials present in the manuscript.
Rabbit Model of Retinoblastoma
Abstract:
We created a rabbit model of retinoblastoma and confirmed the tumor clinically and histopathologically. Seventeen New Zealand rabbits were immunosuppressed with cyclosporin A at doses of 10–15 mg/kg. At day 3, the animals received a 30 μl subretinal injection of 1x10^6 cultured WERI retinoblastoma cells. Digital fundus images were captured before euthanasia, and the eyes were submitted for histopathology. Retinoblastoma cells grew in all the inoculated eyes and established a tumor under the retina and/or in the vitreous. New blood vessels in the tumor were observed starting at week 5. Cuffs of viable tumor cells surrounded the blood vessels with regions of necrosis present at 70–80 μm from nutrient vessels. Occasional tumor seeds in the vitreous histologically exhibited central necrosis. This rabbit model demonstrated similar fundus appearance and pathologic features to human retinoblastoma and may be used as a model to test various routes of drug delivery for retinoblastoma.
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© 2011 Shin Jeong Kang and Hans E. Grossniklaus.
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