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Author Notes:

Address all correspondence to Mob.an Somasundaran, Department of Pediattics, Program in Molecular Medicine, University of Massachusetts Medical Center, "lkvo Biotech, 373 Plantation Street, Worcester, MA 01605.

Subjects:

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Cell Biology
  • CELL BIOLOGY
  • HUMAN-IMMUNODEFICIENCY-VIRUS
  • IMMUNE-DEFICIENCY SYNDROME
  • RAPID COLORIMETRIC ASSAY
  • HTLV-III/LAV ENVELOPE
  • INSITU HYBRIDIZATION
  • DISEASE
  • INDUCTION
  • MECHANISM
  • CONTAINS
  • SURVIVAL

Localization of HIV RNA in mitochondria of infected cells: potential role in cytopathogenicity.

Tools:

Journal Title:

Journal of Cell Biology

Volume:

Volume 126, Number 6

Publisher:

, Pages 1353-1360

Type of Work:

Article | Final Publisher PDF

Abstract:

The intracellular distribution of HIV-1 RNA transcripts in infected cells was studied using in situ hybridization detected by electron microscopy and cellular fractionation. Although viral RNA and core protein could be detected throughout the cytoplasm and nucleus, viral RNA was found in significantly increased amounts in mitochondria relative to the cytoplasm and nucleus. In contrast, cellular poly(A) RNA or viral gag proteins were not increased in the mitochondria. A cell line containing an integrated latent genome that could be induced to express viral RNA after phorbol ester stimulation showed an increase in viral RNA accumulation in mitochondria parallel with the increase in HIV expression levels. Concomitant with HIV expression, there was a decrease in mitochondrial viability. Using immunofluorescent markers to detect probes to HIV RNA transcripts and antibodies to mitochondrial proteins simultaneously in single cells, there was an inverse relationship between the amount of viral RNA and mitochondrial integrity. High levels of viral RNA in mitochondria were found in acutely (but not chronically) infected cells. We propose that HIV RNA import into mitochondria can compromise mitochondrial function.

Copyright information:

© The Rockefeller University Press

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