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Author Notes:

Huabei Jiang: hjiang@bme.ufl.edu

We thank Dr. Andrew Y. Wang at Ocean Nanotech, LLC, for providing magnetic IONPs, and Dr. Malgorzata Lipowska at Emory University for synthesis of NIR 830 dye.

No potential conflicts of interest were disclosed.

Subjects:

Research Funding:

This research project was supported by the following National Institutes of Health (NIH) Grants: NIH R21CA 161384 (Huabei Jiang) and R01CA133722 (Lily Yang).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Oncology
  • Surgery
  • ONCOLOGY
  • SURGERY
  • SURGICAL MARGIN STATUS
  • SENTINEL LYMPH-NODES
  • BREAST-TUMOR MARGINS
  • IN-VIVO
  • PARTIAL MASTECTOMY
  • OPTICAL CONTRAST
  • SCANNING-MIRROR
  • TOMOGRAPHY
  • CANCER
  • SYSTEM

Photoacoustic and Fluorescence Image-Guided Surgery Using a Multifunctional Targeted Nanoprobe

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Journal Title:

Annals of Surgical Oncology

Volume:

Volume 21, Number 5

Publisher:

, Pages 1602-1609

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Purpose: A complete surgical excision with negative tumor margins is the single most important factor in the prediction of long-term survival for most cancer patients with solid tumors. We hypothesized that image-guided surgery using nanoparticle-enhanced photoacoustic and fluorescence imaging could significantly reduce the rate of local recurrence. Methods: A murine model of invasive mammary carcinoma was utilized. Three experimental groups were included: (1) control; (2) tumor-bearing mice injected with non-targeted nanoprobe; and (3) tumor-bearing mice injected with targeted nanoprobe. The surgeon removed the primary tumor following the guidance of photoacoustic imaging (PAI), then inspected the surgical wound and removed the suspicious tissue using intraoperative near-infrared (NIR) fluorescence imaging. The mice were followed with bioluminescence imaging weekly to quantify local recurrence. Results: Nanoprobe-enhanced photoacoustic contrast enabled PAI to map the volumetric tumor margins up to a depth of 31 mm. The targeted nanoparticles provided significantly greater enhancement than non-targeted nanoparticles. Seven mice in the group injected with the targeted nanoprobes underwent additional resections based upon NIR fluorescence imaging. Pathological analysis confirmed residual cancer cells in the re-resected specimens in 5/7 mice. Image-guided resection resulted in a significant reduction in local recurrence; 8.7 and 33.3 % of the mice in the targeted and control groups suffered recurrence, respectively. Conclusions: These results suggest that photoacoustic and NIR intraoperative imaging can effectively assist a surgeon to locate primary tumors and to identify residual disease in real-time. This technology has promise to overcome current clinical challenges that result in the need for second surgical procedures.

Copyright information:

© 2014 Society of Surgical Oncology.

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