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471 Views | 292 Downloads

Author Notes:

Yuru Liu at yuruliu@uic.edu

The authors have nothing to disclose.

Subjects:

Research Funding:

This work was supported by National Institutes of Health grants HL105947-01 (YL), HL07829-16 (AM), HL090152 (AM).

Keywords:

  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics
  • Immunology
  • Issue 92
  • Lung
  • injury
  • pseudomonas
  • pneumonia
  • mouse model
  • alveoli
  • RESPIRATORY-DISTRESS-SYNDROME
  • STEM-CELLS
  • REGENERATION
  • PNEUMONIA

Pseudomonas aeruginosa Induced Lung Injury Model

Tools:

Journal Title:

Journal of Visualized Experiments

Volume:

Volume 2014, Number 92

Publisher:

, Pages e52044-e52044

Type of Work:

Article | Final Publisher PDF

Abstract:

In order to study human acute lung injury and pneumonia, it is important to develop animal models to mimic various pathological features of this disease. Here we have developed a mouse lung injury model by intra-tracheal injection of bacteria Pseudomonas aeruginosa (P. aeruginosa or PA). Using this model, we were able to show lung inflammation at the early phase of injury. In addition, alveolar epithelial barrier leakiness was observed by analyzing bronchoalveolar lavage (BAL); and alveolar cell death was observed by Tunel assay using tissue prepared from injured lungs. At a later phase following injury, we observed cell proliferation required for the repair process. The injury was resolved 7 days from the initiation of P. aeruginosa injection. This model mimics the sequential course of lung inflammation, injury and repair during pneumonia. This clinically relevant animal model is suitable for studying pathology, mechanism of repair, following acute lung injury, and also can be used to test potential therapeutic agents for this disease.

Copyright information:

© 2014, Journal of Visualized Experiments

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