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Author Notes:

Correspondence to: John R Hepler, Email: jhepler@emory.edu

No potential conflicts of interest were disclosed.

Subjects:

Keywords:

  • ARF
  • GAPs (GTPase activating proteins)
  • GEFs (guanine nucleotide exchange factors)
  • GPCRs (G protein coupled receptors)
  • GTPases/G proteins
  • RAB
  • RAS
  • effectors

G protein coupled receptor signaling complexes in live cells.

Tools:

Journal Title:

Cellular Logistics

Volume:

Volume 4

Publisher:

, Pages e29392-e29392

Type of Work:

Article | Final Publisher PDF

Abstract:

Classical models of receptor (GPCR) and G protein (Gαβγ) signaling based on biochemical studies have proposed that receptor stimulation results in G protein activation (Gα-GTP) and dissociation of the heterotrimer (Gα-GTP + Gβγ) to regulate downstream signaling events. Unclear is whether or not there exists freely diffusible, activated Gα-GTP on cellular membranes capable of catalytic signal amplification. Recent studies in live cells indicate that GPCRs serve as platforms for the assembly of macromolecular signaling complexes that include G proteins to support a highly efficient and spatially restricted signaling event, with no requirement for full Gα-GTP and Gβγ dissociation and lateral diffusion within the plasma membrane.

Copyright information:

© 2014 Landes Bioscience

This is an Open Access work distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/).

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