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Author Notes:

Email: iskount@emory.edu

J.P.P. designed the majority of the study. E.V. designed part of the study. I.S. designed part of the study. E.S.E. and J.P.P. maintained the animal colony and carried out all the animal work. J.P.P., E.S.E., E.V., I.S. and M.T.T. performed experiments, analyzed data and prepared the figures. J.W.L. prepared the vaccine coated microneedles. B.P.P. consulted in the in vitro skin experiments and contributed in manuscript editing and relevant citations. J.P.P., M.R.P., R.W.C. and I.S. wrote the manuscript.

All authors discussed the results and commented on the manuscript. All experiments were performed in accordance to Emory University's Institutional Animal Care and Use Committee guidelines.

Subjects:

Research Funding:

The work was supported by U.S. National Institute of Health grant NIH EB012495. We thank Dahnide Taylor-Williams for valuable laboratory technical support and thank Derek O'Hagan, Sushma Kommareddy and their colleagues at Novartis Vaccines and Diagnostics for providing influenza vaccine.

Keywords:

  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics
  • MULTIDISCIPLINARY SCIENCES
  • DERMAL DENDRITIC CELLS
  • EPIDERMAL LANGERHANS CELLS
  • INACTIVATED INFLUENZA-VIRUS
  • IN-VIVO
  • T-CELLS
  • SKIN
  • DELIVERY
  • IMMUNIZATION
  • ANTIGEN
  • MOUSE

A protective role of murine langerin(+) cells in immune responses to cutaneous vaccination with microneedle patches

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Journal Title:

Scientific Reports

Volume:

Volume 4

Publisher:

, Pages 6094-6094

Type of Work:

Article | Final Publisher PDF

Abstract:

Cutaneous vaccination with microneedle patches offers several advantages over more frequently used approaches for vaccine delivery, including improved protective immunity. However, the involvement of specific APC subsets and their contribution to the induction of immunity following cutaneous vaccine delivery is not well understood. A better understanding of the functions of individual APC subsets in the skin will allow us to target specific skin cell populations in order to further enhance vaccine efficacy. Here we use a Langerin-EGFP-DTR knock-in mouse model to determine the contribution of langerin+ subsets of skin APCs in the induction of adaptive immune responses following cutaneous microneedle delivery of influenza vaccine. Depletion of langerin + cells prior to vaccination resulted in substantial impairment of both Th1 and Th2 responses, and decreased post-challenge survival rates, in mice vaccinated cutaneously but not in those vaccinated via the intramuscular route or in non-depleted control mice. Our results indicate that langerin+ cells contribute significantly to the induction of protective immune responses following cutaneous vaccination with a subunit influenza vaccine.

Copyright information:

© 2014, Macmillan Publishers Limited. All rights reserved

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits making multiple copies, distribution, public display, and publicly performance, distribution of derivative works, provided the original work is properly cited. This license requires copyright and license notices be kept intact, credit be given to copyright holder and/or author.

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