About this item:

434 Views | 0 Downloads

Author Notes:

Correspondence: Sarah K. Wise, M.D., Department of Otolaryngology, Emory University, 550 Peachtree St, MOT 9th Floor, Atlanta, GA 30308. Phone: (404) 778-3381. Fax: (404) 686-4540. Email: skmille@emory.edu

Acknowledgments: The authors would like to thank Dr. Porfirio Nava and Dr. Keli Kolegraff for their critical support with immunofluorescence, and Dr. Susan Voss for her assistance in cell culture.

Disclosures: Sarah K. Wise, M.D. discloses an unrestricted Educational Grant from Arthrocare ENT

All other authors have no potential conflicts of interest.

Approval for this study was granted by the Institutional Review Board of Emory University.

Subjects:

Research Funding:

This work was supported in part by the following funding sources: Research in Otolaryngology & Allergy Development (ROAD) Scholarship Program Grant of the AAOA (O.A.H. and S.K.W.)

American Rhinologic Society New Investigator Award (S.K.W.)

Clinical and Translational Science Award Program, National Institutes of Health, National Center for Research Resources KL2 RR025009 and UL1 RR025008 (S.K.W.)

Structure function studies in intestinal epithelial JAM, National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases DK061379 (C.A.P.)

Neutrophil interactions with intestinal epithelial cells, National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases, DK072564 (C.A.P.)

Intestinal epithelial tight junction structure-function, National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases, DK059888 (A.N.)

Short Term Training in Health Professional Schools, National Institutes of Health T35-HL007473 (K.D.B.)

Keywords:

  • House dust mite
  • rhinitis
  • tight junction
  • allergic rhinitis
  • epithelium
  • sinonasal
  • junction adhesion molecule
  • claudin-1

House Dust Mite Der p 1 Effects on Sinonasal Epithelial Tight Junctions

Tools:

Journal Title:

International Forum of Allergy and Rhinology

Volume:

Volume 3, Number 8

Publisher:

, Pages 630-635

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Background Epithelial permeability is highly dependent upon the integrity of tight junctions, cell-cell adhesion complexes located at the apical aspect of the lateral membrane of polarized epithelial cells. We hypothesize that sinonasal epithelial exposure to Der p 1 house dust mite antigen decreases expression of tight junction proteins (TJPs), representing a potential mechanism for increased permeability and presentation of antigens across the sinonasal epithelial layer. Methods Confluent cultured primary human sinonasal epithelial cells were exposed to recombinant Der p 1 antigen versus control, and transepithelial resistance measurements were performed over 24 hours. Antibody staining for a panel of tight junction proteins was examined with immunofluorescence/confocal microscopy and Western blotting. Tissue for these experiments was obtained from 4 patients total. Results Der p 1 exposed sinonasal cells showed a marked decrease in transepithelial resistance when compared to control cells. In addition, results of Western immunoblot and immunofluorescent labeling demonstrated decreased expression of TJPs claudin-1 and junction adhesion molecule-A (JAM-A) in Der p 1 exposed cultured sinonasal cells versus controls. Conclusion Der p 1 antigen exposure decreases sinonasal epithelium TJP expression, most notably seen in JAM-A and claudin-1 in these preliminary experiments. This decreased TJP expression likely contributes to increased epithelial permeability and represents a potential mechanism for transepithelial antigen exposure in allergic rhinitis.

Copyright information:

© 2013 ARS-AAOA, LLC

Export to EndNote