About this item:

496 Views | 0 Downloads

Author Notes:

Address correspondence to: Shawn M. Jobe, Emory Children’s Center, 2015 Uppergate Drive, 4th floor, Atlanta, GA 30068, shawn.jobe@emory.edu, Phone: 404-727-2712, Fax: 404-727-4455

Acknowledgments: H.C. designed and performed the experiments, analyzed the data and wrote the manuscript; T.B.S. performed and assisted in the design of confocal microscopy experiments; L.M. performed experiments; M.B.W. assisted in the design and analysis of confocal microscopy experiments; S.M.J participated in experimental design and data analysis and wrote the manuscript.

The authors declare no competing financial interests.

Subjects:

Research Funding:

This work was supported by grants from the National Institutes of Health/National Heart, Lung, and Blood Institute (R01 HL095858, S.M.J.) and the American Heart Association (Fellow-to-Faculty Transition Award, S.M.J.).

Keywords:

  • Mitochondria
  • calcium
  • phosphatidylserine
  • platelets
  • mPTP

Mitochondrial Calcium and Reactive Oxygen Species Regulate Agonist-Initiated Platelet Phosphatidylserine Exposure

Tools:

Journal Title:

Arteriosclerosis, Thrombosis, and Vascular Biology

Volume:

Volume 32, Number 12

Publisher:

, Pages 2946-2955

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Objective To study the interactions of cytoplasmic calcium (Ca2+cyt) elevation, mitochondrial permeability transition pore (mPTP) formation, and reactive oxygen species (ROS) formation in the regulation of phosphatidylserine (PS) exposure in platelets. Methods and results mPTP formation, but not the degree of Ca2+cyt elevation, was associated with PS exposure in wild-type, CypD null, ionomycin-treated and ROS-treated platelets. In the absence of the mPTP regulator cyclophilin D agonist-initiated mPTP formation and high-level PS exposure were markedly blunted, but Ca2+cyt transients were unchanged. Mitochondrial calcium (Ca2+mit) transients and ROS, key regulators of mPTP formation, were examined in strongly-stimulated platelets. Increased ROS production occurred in strongly-stimulated platelets and was dependent on extracellular calcium entry, but not the presence of CypD. Ca2+mit increased significantly in strongly-stimulated platelets. Abrogation of Ca2+mit entry either by inhibition of the mitochondrial calcium uniporter or mitochondrial depolarization prevented mPTP formation and exposure, but not platelet aggregation or granule release. Conclusions Sustained Ca2+cyt levels are necessary, but not sufficient, for high-level PS exposure in response to agonists. Increased Ca2+mit levels are a key signal initiating mPTP formation and PS exposure. Blockade of Ca2+mit entry allows the specific inhibition of platelet procoagulant activity.

Copyright information:

© 2012, Wolters Kluwer Health

Export to EndNote