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Author Notes:

Corresponding author: Shuming Nie, Email: snie@emory.edu

B.A.K., A.M.S., and S.M. hold patents related to QD synthesis, coating, and use for diagnostics.

The other authors are not aware of any other affiliations, memberships, funding, or financial holdings that might be perceived as affecting the objectivity of this review.

Subjects:

Research Funding:

The authors acknowledge the National Institutes of Health for financial support (grants R01CA163256, RC2CA148265, and HHSN268201000043C).

S.N. and M.D.W. are Distinguished Cancer Scholars of the Georgia Cancer Coalition.

A.M.S. acknowledges the National Cancer Institute Nano-Alliance Program for a Pathway to Independence Award (1K99CA154006).

Keywords:

  • nanotechnology
  • fluorescence imaging
  • cellular dynamics
  • multiplexing
  • cancer detection
  • single-cell analysis

Semiconductor Quantum Dots for Bioimaging and Biodiagnostic Applications

Tools:

Journal Title:

Annual Review of Analytical Chemistry

Volume:

Volume 6, Number 1

Publisher:

, Pages 143-162

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Semiconductor quantum dots (QDs) are light-emitting particles on the nanometer scale that have emerged as a new class of fluorescent labels for chemical analysis, molecular imaging, and biomedical diagnostics. Compared with traditional fluorescent probes, QDs have unique optical and electronic properties such as size-tunable light emission, narrow and symmetric emission spectra, and broad absorption spectra that enable the simultaneous excitation of multiple fluorescence colors. QDs are also considerably brighter and more resistant to photobleaching than are organic dyes and fluorescent proteins. These properties are well suited for dynamic imaging at the single-molecule level and for multiplexed biomedical diagnostics at ultrahigh sensitivity. Here, we discuss the fundamental properties of QDs; the development of next-generation QDs; and their applications in bioanalytical chemistry, dynamic cellular imaging, and medical diagnostics. For in vivo and clinical imaging, the potential toxicity of QDs remains a major concern. However, the toxic nature of cadmium-containing QDs is no longer a factor for in vitro diagnostics, so the use of multicolor QDs for molecular diagnostics and pathology is probably the most important and clinically relevant application for semiconductor QDs in the immediate future.

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© 2013 by Annual Reviews. All rights reserved

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