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Author Notes:

Correspondence to: Dr. Lily Yang; Department of Surgery and Winship Cancer Institute; Emory University School of Medicine; Clinic C, Room C-4088; 1365 C Clifton Road N.E.; Atlanta, Georgia 30322 USA; Tel.: 404.778.4269; Fax: 404.778.5530; lyang02@emory.edu

We would like to thank Dr. Prasanthi Karna for quantitative analysis of K-ras MB labeled fluorescence images. We would also like to thank Dr. Mark Behlke for suggestions on the design of K-ras MBs and survivin MB-Cy3 and for providing MBs and DNA targets during our pilot study for this research project.

We appreciate helpful discussions and collaborations with Drs. Wei Zhou, Andrew Tsourkas, and Gang Bao.


Research Funding:

This research was supported by the funds from the Wallace H. Coulter Foundation, NIH CA80017 and NIH CA095643.


  • molecular imaging
  • molecular beacon
  • K-ras mutation
  • survivin
  • in situ gene expression detection and pancreatic cancer cells

Molecular Beacon Imaging of Tumor Marker Gene Expression in Pancreatic Cancer Cells


Journal Title:

Cancer Biology and Therapy


Volume 4, Number 5


, Pages 561-570

Type of Work:

Article | Post-print: After Peer Review


We have developed a fluorescence imaging-based approach to detect expression of tumor marker genes in pancreatic cancer cells using molecular beacons (MBs). MBs are short hairpin oligonucleotide probes that bind to specific oligonucleotide sequences and produce fluorescent signals. MBs targeting transcripts of two tumor marker genes, mutant K-ras and survivin, were synthesized and their specificity in detection of the expression of those genes in pancreatic cancer cells was examined. We found that K-ras MBs differentially bind to mutant K-ras mRNAs, resulting in strong fluorescent signals in pancreatic cancer cells with specific mutant K-ras genes but not in normal cells or cancer cells expressing either wild type or a different mutation of the K-ras gene. Additionally, MBs targeting survivin mRNA produced a bright fluorescent signal specifically in pancreatic cancer cells. We also demonstrated that MBs labeled with different fluorophores could detect survivin and mutant K-ras mRNAs simultaneously in single cancer cells. Furthermore, we showed that survivin and K-ras MBs have a high specificity in identifying cancer cells on frozen sections of pancreatic cancer tissues. In conclusion, molecular beacon-based imaging of expression of tumor marker genes has potential for the development of novel approaches for the detection of pancreatic cancer cells.

Copyright information:

©2005 Landes Bioscience

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