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Author Notes:

Address correspondence to: Dr. Hongjie Yuan, Department of Pharmacology, Rollins Research Center, 1510 Clifton Road, Emory University, Atlanta, GA 30322. Tel: 404-727-1375; Fax: 404-727-0365; hyuan@emory.edu

Subject:

Research Funding:

National Institute of Neurological Disorders and Stroke : NINDS

This work was supported by NIH (NS036654, SFT), the Michael J. Fox Foundation (SFT), the Lundbeck Foundation (KBH), and the Villum Kann Rasmussen Foundation (KBH).

Keywords:

  • ionotropic glutamate receptors
  • electrophysiological recordings
  • structure-activity
  • Xenopus Oocytes
  • single channel recordings
  • GluN2
  • GluN1

Control of N-methyl-D-aspartate Receptor Function by the NR2 Subunit Amino-Terminal Domain

Tools:

Journal Title:

Journal of Neuroscience Nursing

Volume:

Volume 29, Number 39

Publisher:

, Pages 12045-12058

Type of Work:

Article | Post-print: After Peer Review

Abstract:

NMDA receptors comprised of different NR2 subunits exhibit strikingly unique biophysical and pharmacological properties. Here we report that the extracellular amino-terminal domain (ATD) of the NR2 subunit controls pharmacological and kinetic properties of recombinant NMDA receptors, such as agonist potency, deactivation time course, open probability (POPEN), and mean open/shut duration. Using ATD deletion mutants of NR2A, NR2B, NR2C, NR2D and chimeras of NR2A and NR2D with interchanged ATD (NR2A-(2D-ATD) and NR2D-(2A-ATD)), we show that the ATD contributes to the low glutamate potency of NR2A-containing NMDA receptors and the high glutamate potency of NR2D-containing receptors. The ATD influences the deactivation time courses of NMDA receptors, as removal of the ATD from NR2A slows the deactivation rate, while removal of the ATD from NR2B, NR2C and NR2D accelerates the deactivation rate. Open probability also is influenced by the ATD. Removal of the ATD from NR2A or replacement of the NR2A-ATD with that of NR2D decreases POPEN in single channel recordings from outside-out patches of HEK 293 cells. By contrast, deletion of the ATD from NR2D or replacement of the NR2D ATD with that of NR2A increases POPEN and mean open duration. These data demonstrate the modular nature of NMDA receptors and show that the ATD of the different NR2 subunits plays an important role in fine-tuning the functional properties of the individual NMDA receptor subtypes.

Copyright information:

© 2009 Society for Neuroscience

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