Chronic, Constant-Rate, Gastric Drug Infusion in Nontethered Rhesus Macaques (Macaca mulatta)

• Authors: Karen Strait, Emory University; Jack L Orkin, Emory University; Daniel C Anderson, Emory University; E Christopher Muly, Emory University
• Journal Title: Journal of the American Association for Laboratory Animal Science : JAALAS
• Volume: Volume 49, Number 2
• Publisher: American Association for Laboratory Animal Science | 2010-03, Pages 207-214
• Type of Work: Article | Post-print: After Peer Review
• Subjects: Health Sciences, Pathology; Agriculture, Animal Pathology; Psychology, Behavioral

Abstract

As part of a study of antipsychotic drug treatment in monkeys, we developed a technique to provide chronic, constant-rate, gastric drug infusion in nontethered rhesus macaques. This method allowed us to mimic the osmotic release oral delivery system currently used in humans for continuous enteral drug delivery. Rhesus macaques (n = 5) underwent gastric catheter placement by laparotomy. After the catheters were secured to the stomach, the remaining catheter length was exited through the lateral abdomen, tunneled subcutaneously along the back, and connected to a 2-mL osmotic pump enclosed in a subcutaneous pocket. Osmotic pumps were changed every 2 to 4 wk for 1 y and remained patent for the duration of the study. Four complications (including cutting of the catheter, incisional dehiscence at the pump site, and loss of 1 catheter into the abdominal cavity requiring catheter replacement) occurred among the 80 pump changes performed during the yearlong study. At necropsy, histopathologic examination of the catheter implant sites revealed mild changes consistent with a foreign-body reaction. Our results indicate that the gastric catheter and osmotic pump system was well tolerated in rhesus macaques for as long as 12 mo after placement and suggest that this system will be an attractive option for use in studies that require chronic, constant-rate, gastric drug infusion in nontethered monkeys.