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Author Notes:

The first two authors contributed equally to this publication.

Correspondence to: John M. Nickerson, Ph.D., Department of Ophthalmology, Emory University, Room B5602, 1365B Clifton Road, NE, Atlanta, GA, 30322; Phone: (404) 778-4411; FAX: (404) 778-2231; email: litjn@emory.edu

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Research Funding:

This work was supported by grants from the National Eye Institute (R03 EY13986, R01 EY09378, P30 EY6360), Research to Prevent Blindness, Inc., and the Foundation Fighting Blindness.

Quantifying DNA concentrations using fluorometry: A comparison of fluorophores

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Journal Title:

Molecular Vision

Volume:

Volume 2002, Number 8

Publisher:

, Pages 416-421

Type of Work:

Article | Final Publisher PDF

Abstract:

Detection of low concentrations of DNA is important in vision research because many animal models only provide scant samples of ocular tissue. Quantitative analysis of low concentrations of double stranded DNA is now feasible using fluorometry with newer fluorophores. This technique offers a rapid way to evaluate the DNA content of samples based on the measurement of fluorescence enhancement emitted by fluorophore-bound DNA and is more sensitive than absorption spectrometry. The purpose of this study was to compare the sensitivity of several different fluorophores for measuring DNA concentrations by fluorometry. Based on our studies, we conclude that SYBR Green I and PicoGreen are substantially more sensitive for quantifying DNA concentrations than ethidium bromide and Hoechst 33258.

Copyright information:

© 2002 Molecular Vision

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommerical-NoDerivs 3.0 Unported License ( http://creativecommons.org/licenses/by-nc-nd/3.0/), which permits distribution, public display, and publicly performance, making multiple copies, provided the original work is properly cited. This license requires copyright and license notices be kept intact, credit be given to copyright holder and/or author. This license prohibits exercising rights for commercial purposes.

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