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Author Notes:

Author for correspondence (blevin@emory.edu).

Present address P.O.: Institute of Integrative Biology, ETH Zürich, Universitätstrasse 16, 8092 Zürich, Switzerland.


Research Funding:

This investigation was supported by grants from the US National Institutes of Health, AI40662 (B.R.L.) and GM091875 (B.R.L.).


  • cholera
  • bacteriophage
  • population dynamics
  • evolution
  • Vibrio cholerae

An experimental study of the population and evolutionary dynamics of Vibrio cholerae O1 and the bacteriophage JSF4


Journal Title:

Proceedings of the Royal Society B: Biological Sciences


Volume 277, Number 1698


, Pages 3247-3254

Type of Work:

Article | Post-print: After Peer Review


Studies of Vibrio cholerae in the environment and infected patients suggest that the waning of cholera outbreaks is associated with rise in the density of lytic bacteriophage. In accordance with mathematical models, there are seemingly realistic conditions where phage predation could be responsible for declines in the incidence of cholera. Here, we present the results of experiments with the El Tor strain of V. cholerae (N16961) and a naturally occurring lytic phage (JSF4), exploring the validity of the main premise of this model: that phage predation limits the density of V. cholerae populations. At one level, the results of our experiments are inconsistent with this hypothesis. JSF4-resistant V. cholerae evolve within a short time following their confrontation with these viruses and their populations become limited by resources rather than phage predation. At a larger scale, however, the results of our experiments are not inconsistent with the hypothesis that bacteriophage modulate outbreaks of cholera. We postulate that the resistant bacteria that evolved play an insignificant role in the ecology or pathogenicity of V. cholerae. Relative to the phage-sensitive cells from whence they are derived, the evolved JSF4-resistant V. cholerae have fitness costs and other characters that are likely to impair their ability to compete with the sensitive cells in their natural habitat and may be avirulent in human hosts. The results of this in vitro study make predictions that can be tested in natural populations of V. cholerae and cholera-infected patients.

Copyright information:

© 2010 The Royal Society

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