About this item:

442 Views | 0 Downloads

Author Notes:

Reprint requests available from the corresponding author: Cassandra D. Josephson, MD Children’s Healthcare of Atlanta Department of Pathology 1405 Clifton Road NE Atlanta, GA 30322 Phone: 404-785-4553 Fax: 404-785-1370 cjoseph@emory.edu

Subject:

Research Funding:

National Heart, Lung, and Blood Institute : NHLBI

The manuscript is supported in part by NIH awards HL-07-001 Pediatric Transfusion Medicine Academic Career Award and HL086773-01 Program Project Grant in Transfusion Medicine

Keywords:

  • residual apheresis platelet volumes storage
  • platelet metabolism and function
  • pediatric transfusion of platelets

Effects of Storage Duration and Volume on the Quality of Leukoreduced Apheresis-derived Platelets: Implications for Pediatric Transfusion Medicine

Tools:

Journal Title:

Transfusion

Volume:

Volume 50, Number 10

Publisher:

, Pages 2193-2198

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Background Platelet storage adversely affects platelet structure and function in vitro, and is associated with decreased platelet recovery and function in vivo. In pediatric transfusion medicine, it is not uncommon for small residual volumes to remain in parent units following aliquot preparation of leukoreduced apheresis-derived platelets (LR-ADP). However, limited data exists regarding the impact of storage on residual small volume LR-ADP. Study Design and Methods Standard metabolic testing was performed on residual volumes of LR-ADP following aliquot removal and platelet aggregometry using a dual agonist of ADP and collagen was performed on stored, small volume aliquots (10-80 ml) created from an in vitro model of platelet storage. Results 77 LR-ADP underwent metabolic (n=67) or metabolic and aggregation (n=10) studies. All products maintained a pH > 6.89 throughout storage. Lactate and pCO2 increased proportionally with longer storage time. Regardless of acceptable metabolism during storage, aggregation in 10-20 ml aliquots was impaired by day 4 and aliquots less than 40 ml demonstrated the most dramatic decrease in aggregation from baseline. Conclusions Despite maintenance of acceptable metabolic conditions, residual volumes of LR-ADP develop impaired aggregation in vitro that may adversely affect platelet survival and function in vivo. At volumes below 40 ml, LR-ADP revealed reduced aggregation. As a result, it is recommended to monitor and record volumes of LR-ADP used for pediatric transfusion. Moreover, once LR-ADP attain a volume of 50 ml or less on day 4 or 5 of storage, consider discarding these products until their in vivo efficacy can be studied.

Copyright information:

© 2010 American Association of Blood Banks

Export to EndNote