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Author Notes:

Correspondence: Xiaoping Hu, Address: 101 Woodruff Circle, Suite 2001, Atlanta, GA 30322; Fax: (404) 712-2707; Phone: (404) 712-2615; Email: xhu3@emory.edu

Acknowledgements: We thank the reviewers for their valuable suggestions, which significantly improved the quality of the manuscript.

We thank Dr. Mar Sanchez for kindly providing us with the four in vivo macaque diffusion MRI data.


Research Funding:

This work was supported by NIH 5P01 AG026423-03, NIH 2P51RR0001 65-51, R01 MH084068-01A1, NIH/NIMH P50 MH078105, NIH/NIDA P30 DA023920.


  • connectivity
  • diffusion
  • macaque
  • phantom
  • transcallosal motor fibers

Quantitative Assessment of a Framework for Creating Anatomical Brain Networks via Global Tractography

Journal Title:



Volume 61, Number 4


, Pages 1017-1030

Type of Work:

Article | Post-print: After Peer Review


Interregional connections of the brain measured with diffusion tractography can be used to infer valuable information regarding both brain structure and function. However, different tractography algorithms can generate networks that exhibit different characteristics, resulting in poor reproducibility across studies. Therefore, it is important to benchmark different tractography algorithms to quantitatively assess their performance. Here we systematically evaluated a newly introduced tracking algorithm, global tractography, to derive anatomical brain networks in a fiber phantom, 2 post-mortem macaque brains, and 20 living humans, and compared the results with an established local tracking algorithm. Our results demonstrated that global tractography accurately characterized the phantom network in terms of graph-theoretic measures, and significantly outperformed the local tracking approach. Results in brain tissues (post-mortem macaques and in vivo humans), however, showed that although the performance of global tractography demonstrated a trend of improvement, the results were not vastly different than that of local tractography, possibly resulting from the increased fiber complexity of real tissues. When using macaque tracer-derived connections as the ground truth, we found that both global and local algorithms generated non-random patterns of false negative and false positive connections that were probably related to specific fiber systems and largely independent of the tractography algorithm or tissue type (post-mortem vs. in vivo) used in the current study. Moreover, a close examination of the transcallosal motor connections, reconstructed via either global or local tractography, demonstrated that the lateral transcallosal fibers in humans and macaques did not exhibit the denser homotopic connections found in primate tracer studies, indicating the need for more robust brain mapping techniques based on diffusion MRI data.

Copyright information:

© 2012 Elsevier Inc. All rights reserved.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommerical-NoDerivs 3.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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