About this item:

744 Views | 0 Downloads

Author Notes:

Address for reprint requests and other correspondence: T. O. Ilori, Renal Div., Dept. of Medicine, Emory Univ., 338 Woodruff Memorial Bldg., 1639 Pierce Dr., Atlanta, GA 30322 (e-mail: tilori@emory.edu).

Subject:

Research Funding:

This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases R01-DK41707, RO1-DK89828, and T32-DK007656.

Keywords:

  • FK506
  • phosphatase
  • urea transport
  • calyculin
  • PP2B

Acute calcineurin inhibition with tacrolimus increases phosphorylated UT-A1

Tools:

Journal Title:

American Journal of Physiology - Renal Physiology

Volume:

Volume 302, Number 8

Publisher:

, Pages F998-F1004

Type of Work:

Article | Post-print: After Peer Review

Abstract:

UT-A1, the urea transporter present in the apical membrane of the inner medullary collecting duct, is crucial to the kidney's ability to concentrate urine. Phosphorylation of UT-A1 on serines 486 and 499 is important for plasma membrane trafficking. The effect of calcineurin on dephosphorylation of UT-A1 was investigated. Inner medullary collecting ducts from Sprague-Dawley rats were metabolically labeled and treated with tacrolimus to inhibit calcineurin or calyculin to inhibit protein phosphatases 1 and 2A. UT-A1 was immunoprecipitated, electrophoresed, blotted, and total UT-A1 phosphorylation was assessed by autoradiography. Total UT-A1 was determined by Western blotting. A phospho-specific antibody to pser486-UT-A1 was used to determine whether serine 486 can be hyperphosphorylated by inhibiting phosphatases. Inhibition of calcineurin showed an increase in phosphorylation per unit protein at serine 486. In contrast, inhibition of phosphatases 1 and 2A resulted in an increase in UT-A1 phosphorylation but no increase in pser486-UT-A1. In vitro perfusion of inner medullary collecting ducts showed tacrolimus-stimulated urea permeability consistent with stimulated urea transport. The location of phosphorylated UT-A1 in rats treated acutely and chronically with tacrolimus was determined using immunohistochemistry. Inner medullary collecting ducts of the acutely treated rats showed increased apical membrane association of phosphorylated UT-A1 while chronic treatment reduced membrane association of phosphorylated UT-A1. We conclude that UT-A1 may be dephosphorylated by multiple phosphatases and that the PKA-phosphorylated serine 486 is dephosphorylated by calcineurin. This is the first documentation of the role of phosphatases and the specific site of phosphorylation of UT-A1, in response to tacrolimus.

Copyright information:

© 2012 the American Physiological Society

Export to EndNote