A Dual-Readout F2 Assay That Combines Fluorescence Resonance Energy Transfer and Fluorescence Polarization for Monitoring Bimolecular Interactions

• Authors: Yuhong Du, Emory University; Zaneta Nikolovska-Coleska, University of Michigan; Min Qui, Emory University; Lian Li, Emory University; Iestyn Lewis, Emory University; Raymond J Dingledine, Emory University; Jeanne A. Stuckey, University of Michigan; Krzysztof Krajewski, National Institutes of Health; Peter P. Roller, National Institutes of Health; Shaomeng Wang, University of Michigan Comprehensive Cancer Center; Haian Fu, Emory University
• Journal Title: ASSAY and Drug Development Technologies
• Volume: Volume 9, Number 4
• Publisher: Mary Ann Liebert | 2011-08, Pages 382-393
• Type of Work: Article | Post-print: After Peer Review
• Publisher DOI: http://doi.org/10.1089/adt.2010.0292
• Subjects: Health Sciences, Pharmacology; Health Sciences, Pathology; Chemistry, Biochemistry

Abstract

Förster (fluorescence) resonance energy transfer (FRET) and fluorescence polarization (FP) are widely used technologies for monitoring bimolecular interactions and have been extensively used in high-throughput screening (HTS) for probe and drug discovery. Despite their popularity in HTS, it has been recognized that different assay technologies may generate different hit lists for the same biochemical interaction. Due to the high cost of large-scale HTS campaigns, one has to make a critical choice to employee one assay platform for a particular HTS. Here we report the design and development of a dual-readout HTS assay that combines two assay technologies into one system using the Mcl-1 and Noxa BH3 peptide interaction as a model system. In this system, both FP and FRET signals were simultaneously monitored from one reaction, which is termed “Dual-Readout F2 assay” with F2 for FP and FRET. This dual-readout technology has been optimized in a 1,536-well ultra-HTS format for the discovery of Mcl-1 protein inhibitors and achieved a robust performance. This F2 assay was further validated by screening a library of 102,255 compounds. As two assay platforms are utilized for the same target simultaneously, hit information is enriched without increasing the screening cost. This strategy can be generally extended to other FP-based assays and is expected to enrich primary HTS information and enhance the hit quality of HTS campaigns.